排序方式: 共有11条查询结果,搜索用时 15 毫秒
1.
Ghalandari Mohammad Irandoost Shahrestani Misagh Maleki Akbar Safdari Shadloo Mostafa El Haj Assad Mamdouh 《Journal of Thermal Analysis and Calorimetry》2021,145(4):1837-1848
Journal of Thermal Analysis and Calorimetry - Heat exchangers are applicable in different industries and technologies, and their performance is influenced by different parameters. In addition to... 相似文献
2.
Yuqing Ke Behafarid Ghalandari Shiyi Huang Sijie Li Chengjie Huang Xiao Zhi Daxiang Cui Xianting Ding 《Chemical science》2022,13(7):2050
The CRISPR–Cas12a system has been widely applied to genome editing and molecular diagnostics. However, off-target cleavages and false-positive results remain as major concerns in Cas12a practical applications. Herein, we propose a strategy by utilizing the 2′-O-methyl (2′-OMe) modified guide RNA (gRNA) to promote the Cas12a''s specificity. Gibbs free energy analysis demonstrates that the 2′-OMe modifications at the 3′-end of gRNA effectively suppress the Cas12a''s overall non-specific affinity while maintaining high on-target affinity. For general application illustrations, HBV genotyping and SARS-CoV-2 D614G mutant biosensing platforms are developed to validate the enhanced Cas12a''s specificity. Our results indicate that the 2′-OMe modified gRNAs could discriminate single-base mutations with at least two-fold enhanced specificity compared to unmodified gRNAs. Furthermore, we investigate the enhancing mechanisms of the 2′-OMe modified Cas12a systems by molecular docking simulations and the results suggest that the 2′-OMe modifications at the 3′-end of gRNA reduce the Cas12a''s binding activity to off-target DNA. This work offers a versatile and universal gRNA design strategy for highly specific Cas12a system development.This study illustrates that 2′-O-methyl modified gRNAs improve the specificity of the CRISPR–Cas12a system (mg-CRISPR) via suppressing the Cas12a''s affinity to off-target DNA and provides an efficient strategy for high-specificity gRNA design. 相似文献
3.
Dr. Shuang Zhang Dr. Behafarid Ghalandari Dr. Aiting Wang Dr. Sijie Li Dr. Youming Chen Dr. Qingwen Wang Dr. Lai Jiang Prof. Xianting Ding 《Angewandte Chemie (International ed. in English)》2023,62(45):e202309806
Mass spectrometry has emerged as a mainstream technique for label-free proteomics. However, proteomic coverage for trace samples is constrained by adsorption loss during repeated elution at sample pretreatment. Here, we demonstrated superparamagnetic composite nanoparticles functionalized with molecular glues (MGs) to enrich proteins in trace human biofluid. We showed high protein binding (>95 %) and recovery (≈90 %) rates by anchor-nanoparticles. We further proposed a Streamlined Workflow based on Anchor-nanoparticles for Proteomics (SWAP) method that enabled unbiased protein capture, protein digestion and pure peptides elution in one single tube. We demonstrated SWAP to quantify over 2500 protein groups with 100 HEK 293T cells. We adopted SWAP to profile proteomics with trace aqueous humor samples from cataract (n=15) and wet age-related macular degeneration (n=8) patients, and quantified ≈1400 proteins from 5 μL aqueous humor. SWAP simplifies sample preparation steps, minimizes adsorption loss and improves protein coverage for label-free proteomics with previous trace samples. 相似文献
4.
Sara Nourizadeh Adeleh Divsalar Mina Fekri Behafarid Ghalandari Mahbubeh Eslami-Moghadam Ali Akbar Saboury Saba Abbasi 《Journal of fluorescence》2018,28(2):551-559
In the present study, the biological activities of a new synthesized Pt(II)-complex, 2,2′ bipyridinphenyl isopentylglycin Pt(II) nitrate was investigated via its interaction with the most important blood carrier protein of human serum albumin (HSA), using fluorescence and Far-UV circular dichroism (CD) spectroscopic techniques and also molecular docking. Moreover, cytotoxicity activity of the complex was studied against breast cancer cell line of MDA MB231 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The Pt(II)-complex has a strong ability to quench the intrinsic fluorescence of HSA through a static quenching mechanism. According fluorescence quenching data, the binding parameters of the interaction were calculated and showed that hydrophobic interaction has an important role. The molecular docking results in coherent with fluorescence measurements illustrated that Pt(II) complex can bind to HSA at one position that located in the hydrophobic cavity of groove between drug site I and II. Also, experimental data on driving force in binding site was confirmed whereas theoretical results demonstrated Pt(II) complexinteract to HSA by hydrophobic interaction. Far-UV-CD results showed that Pt(II)-complex induced an increasing in the content of α-helical structure of the protein and stabilized it. Also, MTT assay represented growth inhibitory effect of the complex toward the breast cancer cell line. 相似文献
5.
Shahraki Shahdabadi Reza Maleki Akbar Haghighat S. Ghalandari Mohammad 《Journal of Thermal Analysis and Calorimetry》2021,145(4):2105-2122
Journal of Thermal Analysis and Calorimetry - Biomass is one of the most convenient types of renewable energy resources around the world. Densely populated areas all over the globe produce a vast... 相似文献
6.
Propagation characteristics of a high-power electromagnetic wave through an inhomogeneous magnetized plasma is investigated. Considering the momentum transfer equations for electrons and ions and taking into account the ponderomotive force, the distribution of electron density and dielectric permittivity are obtained. Using non-linear dielectric permittivity and Maxwell's equations in the absence of external current and charge densities, non-linear wave equations are achieved. The results indicate that the external static magnetic field can modify the profiles of both the electric and magnetic fields. It is also shown that the external static magnetic field enhances the amplitude of the electron density and the non-linear dielectric permittivity. 相似文献
7.
M. Solaimani B. Farnam M. Ghalandari S. Z. SeyedShirazi 《Optical and Quantum Electronics》2018,50(2):114
In this work, we have studied the wave localization in a two dimensional parabolic periodic refractive index profile. Our calculations have been performed by developing a 4th order Runge–Kutta method. Effects of different parameters in refractive index profile and incident wave shape on the wave intensity and shape in the future are shown. Effects of the mentioned parameters on localization degree and total momentum of the system are also investigated. We find different parameters change intervals within which the excitation disperses. Thus no bound state is possible. Finally, we show when a bound state is present. 相似文献
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9.
Ali Afrasiabi Gholam Hossein Riazi Ali Dadras Elaheh Tavili Behafarid Ghalandari Ali Naghshineh Hamid Mobasheri Shahin Ahmadian 《Journal of the Iranian Chemical Society》2014,11(5):1295-1304
Nowadays exposure to extremely low frequency electromagnetic fields (ELF-EMFs) is an unavoidable fact in human societies. In spite of destructive effects of ELF-EMF such as impression on short-term memory and cognitive performances, neuroprotective and neurogeneration effects of ELF-EMF have been reported. On the other hand, microtubule (MT) proteins, the dynamic cytoskeleton proteins that are mostly located in axons, are able to produce local EMFs and possess key role in memory formation and consciousness. Therefore, MTs have strong potential to be affected by exogenous ELF-EMFs. In the present study, decline of MT polymerization was shown by transmission electron microscopy images and turbidimetry method after exposure to ELF-EMFs with 50, 100, and 217 Hz frequencies at 0.2 mT intensity. Circular dichroism and 8-anilino-1-naphthalenesulfonate (ANS) fluorometry were used to demonstrate the MT secondary and third structural changes. Decrement of MT polymerization through disruption of tubulin native structure, and increasing the β-sheets and random coils were observed in all exposure conditions. Therefore, ELF-EMFs with 50, 100, and specially 217 Hz frequencies at 0.2 mT could lead to disruption of MT functions in the neural cells. 相似文献
10.
Ghalandari Mohammad Forootan Fard Habib Komeili Birjandi Ali Mahariq Ibrahim 《Journal of Thermal Analysis and Calorimetry》2021,144(5):1999-2008
Journal of Thermal Analysis and Calorimetry - Carbon dioxide emission of countries is deeply dependent on the energy system. Share of different energy resources in primary energy consumption of the... 相似文献