Analysis of urinary nucleosides. I. Optimisation of high performance liquid chromatography/electrospray mass spectrometry

In order to optimise the analysis of urinary nucleosides by high performance liquid chromatography/mass spectrometry (HPLC/MS), the HPLC separation of these compounds was performed at different 'flow rates' and 0.2mL/min was found to give both a better separation and ionisation. The ionisation conditions were optimised to give the best intensity of the molecules quasi-molecular ions. The ion distribution profile and ionisation in both positive and negative mode were examined and the detection of the protonated molecule in positive mode chosen for further analysis. The limits of detection of the method developed are reported and representative LC/MS and LC/MS/MS spectra shown. Typical urinary nucleoside chromatograms are presented.     

Capillary and packed column SFC/MS

A frit restrictor interface for capillary column supercritical fluid chromatography/mass spectrometry (SFC/MS) has been constructed and used for the analysis of high boiling point alkanes. Packed column SFC/MS is described using both a moving belt liquid chromatographic/mass spectrometric interface and a thermospray source in the filament-on mode.     

Field desorption mass spectra of involatile sulphonic acids,sulphonates and dyestuff materials

Abstract-Useful field desorption mass spectra can be obtained from involatile sulphonic acids and sulphonates, even when large dyestuff molecules are involved. Unusual cluster ions are commonly found, which can be singly or doubly charged. Most ions can be assigned, however, and the great potential of field desorption mass spectrometry in the identification of these involatile species is clearly established.     

A mass spectrometric investigation of the reduction product of 3H-1,2-benzodithiole-3-thione

A combination of electron impact, chemical ionization, field desorption and field ionization mass spectrometry has been employed to demonstrate that the LAH reduction product of 3H-1,2-benzodithiole-3-thione is the dimer, dibenzo[c,h]-3H,10H-dihydro-1,2,8,9-tetrathiecine.     

Conservative methods for the Toda lattice equations

We are concerned with the numerical integration of the Todalattice equations by using different conservative methods. Numericalexperiments suggest that the global error for isospectral schemesdecreases exponentially with time but it is almost constantfor either symplectic or more general integrators. We providea theoretical explanation for these experimental findings.