DETECTION OF DNA-PSORALEN PHOTOADDUCTS in situ

Abstract— An immunological method, with the use of specific immune serum, has been developed for detection of 8-methoxypsoralen (8-MOP) photoadducts to DNA, formed in situ in cell nuclei, after combined treatment with 8MOP and UV-A irradiation (Zarçbska et al. , 1978). Lymphocytes fixed on slides or in suspension, and cryostat sections of different mammalian tissues, served as antigenic substrate, after treatment with 8-MOP and UV-A in vitro. Specific fluorescence in these substrates was detected in the nuclei after treatment with 30 ˜ 140 kJ/m² UV-A in the presence of 0.1-0.3 μg/cm² 8-MOP. PHA-stimulated-lymphocytes appeared to be the most sensitive substrate.
However, hairless mice treated with high doses of UV-A in vivo , 70 ˜ 360 kJ/m² did not reveal a specific fluorescence of epidermal nuclei, unless a high local concentration of 8-MOP was attained.
The apparent discrepancy in the level of photoadduct detection between the in vitro and in vivo treated specimens was explained by the low number of DNA-8-MOP-photoadducts formed in vivo under these experimental conditions. The relevance of these findings to the role of DNA-8-MOP-photoadducts formed during PUVA photochemotherapy is discussed.     

Activation and stabilization of enzymes entrapped into reversed micelles

Observations of the activity of two hydrolyzing enzymes—protease and α-amylase—entrapped inside the reversed micelles formed by surfactants in hexane, benzene, and cyclohexane are reported. The surfactants chosen for this study are: Tween 80, a nonionic surfactant, Cetyl pyridinium chloride, a cationic surfactant, and two anionic surfactants, sodium lauryl sulfate and Aerosol OT. Tween 80 enhances the activity of both protease and α-amylase. Sodium lauryl sulfate and Aerosol OT, which are ionic surfactants, enhance the activity of protease, but inhibit the activity of α-amylase. Cetyl pyridinium chloride, however, enhances the activity of α-amylase, but inhibits the activity of protease. Enhanced activity is generally severalfold greater in comparison to the activity observed in the usual aqueous system in the absence of reversed micelles. It has also been observed that the enhanced activity of the enzymes entrapped inside the reversed micelles remains preserved for a much longer period of time in comparison to the activity in the usual aqueous systems. These observations, which support the view that with proper choice of surfactant and the organic solvent, reversed micelles act like a microreactor that provides a favorable aqueous microenvironment for enzyme activity, have biotechnological overtones.     

Effect of shodhana (purification process) on guggulsterone E and Z in Commiphora mukul

JPC – Journal of Planar Chromatography – Modern TLC - Commiphora mukul of the family Burseraceae, commonly known as guggul, is commonly used worldwide. The major phytoconstituents...     

Nonlinear Stability of Nonstationary Cross-Flow Vortices in Compressible Boundary Layers

The nonlinear evolution of long-wavelength non stationary cross-flow vortices in a compressible boundary layer is investigated; the work extends that of Gajjar [1] to flows involving multiple critical layers. The basic flow profile considered in this paper is that appropriate for a fully three-dimensional boundary layer with O(1) Mach number and with wall heating or cooling. The governing equations for the evolution of the cross-flow vortex are obtained, and some special cases are discussed. One special case includes linear theory, where exact analytic expressions for the growth rate of the vortices are obtained. Another special case is a generalization of the Bassom and Gajjar [2] results for neutral waves to compressible flows. The viscous correction to the growth rate is derived, and it is shown how the unsteady nonlinear critical layer structure merges with that for a Haberman type of viscous critical layer.     

Subtle white matter volume differences in children treated for medulloblastoma with conventional or reduced dose craniospinal irradiation

Medulloblastoma is the most common malignant brain tumor in children, and approximately seventy percent of average-risk patients will achieve long-term survival. Craniospinal irradiation (CSI), combined with chemotherapy and surgery, is currently the mainstay of treatment but places children who survive at risk for serious neurocognitive sequelae. These sequelae are intensified with a younger age at treatment, greater elapsed time following treatment, and an increased radiation dose. Many newer treatment approaches have attempted to address this problem by reducing the dose of the CSI component of radiation therapy while maintaining the current survival rates. This study evaluates longitudinal MR imaging during therapy to assess the impact of the two CSI doses (conventional [36 Gy] and reduced [23.4 Gy]) on normal appearing white matter volumes (NAWMV) evaluated in a single index slice. Twenty-six children and young adults at least three years of age enrolled on an institutional protocol for newly diagnosed, previously untreated primary medulloblastoma had at least four MR examinations over a minimum nine month period following CSI. These serial volumes were evaluated as a function of time since CSI in three analyses: 1) all subjects, 2) subjects stratified by age at CSI, and 3) subjects stratified by CSI dose. The first analysis demonstrated that medulloblastoma patients treated with CSI have a significant loss of NAWMV in contradistiction to normally expected maturation. Stratifying the patients by age at CSI found no significant differences in the rate of NAWMV loss. The final analysis stratified the patients by CSI dose and revealed that the rate of NAWMV loss was 23% slower in children receiving reduced-dose. Serial quantitative MR measures of NAWMV may provide a neuroanatomical substrate for assessing functional impact of CSI on normal brain function following treatment for medulloblastoma.     

The upper-branch stability of compressible boundary-layer flows

The upper-branch linear and nonlinear stability of compressible boundary-layer flows is studied using the approach of Smith and Bodonyi (1982) for a similar incompressible problem. Both pressure gradient boundary layers and Blasius flow are considered with and without heat transfer and the neutral eigenrelations incorporating compressibility effects are explicitly obtained. The compressible nonlinear viscous critical-layer equations are derived and solved numerically and the results indicate some solutions with positive phase shift across the critical layer. Various limiting cases are investigated including the case of much larger disturbance amplitudes and this indicates the structure for the strongly nonlinear critical layer of the Benney-Bergeron (1969) type. Finally, we also show how a match with the inviscid neutral inflexional modes arising from the generalized inflexion-point criterion is achieved.J. Cole is grateful to the Science and Engineering Research Council of Great Britain for financial support. J. Gajjar gratefully acknowledges the support of ICOMP, NASA Lewis Research Center, Cleveland, Ohio, where part of this work was done, and he is also grateful to the Computation Center at Iowa State University for a grant which enabled the numerical work in the paper to be completed. The permanent address of the authors is the Mathematics Department, Exeter University, Exeter EX4 4QE, England.     

Asphericity in the Fermi Surface and Fermi Energy of Na-K, Na-Rb and Na-Cs Binary Alloys

Detailed theoretical investigations into asphericity in the Fermi surface (FS) and Fermi energy (FE) ofNa1_xKx, Na1_xRbx, and Na1_xCsx binary solid solutions are carried out for the first time. The alloying behavior ofthe K, Rb, and Cs with the Na generates the Fermi surface distortion (FSD) of bce simple metals. The FS of Na-K,Na-Rb, and Na-Cs solid solution is a distorted sphere with the largest deviation along [110]. We have found that theimpact of local-field correction function on FSD is maximun at [100] point and minimum at [111] point. The exchangeand correlation effect is found to suppress the value of FE.     

Thermal rectification and thermal resistive phase cross over in exponential mass graded materials

Concept of the functional graded materials (FGMs) has been explored by consideringexponential mass variation along the chain of anharmonic oscillators in the study of heattransport at low dimensions. This exponential distribution of mass along the space invokesthe diffusion of phonons transport which results to temperature gradient, asymmetric heatflow, thermal rectification and cross over between positive differential thermalresistance (PDTR) and negative differential thermal resistance (NDTR) in one-dimensional(1D) exponential mass graded chain. The temperature dependence thermal rectificationachieved is 4?74% and also predicted that the thermal rectification can be controlled bytuning the higher and lower average temperature limits of two thermal reservoirs. It isalso seen that in FGMs, the thermal conductivity does not change drastically against theaverage temperature of two heat baths. The cross over between PDTR and NDTR can be tunedeither by mass ratio of one dimensional (1D) exponential mass graded anharmonic chainand/or by temperature difference between two heat baths. The figure of merit of the 1Dstructure can also be tuned by mass gradient, the higher mass gradient material will workas the potential candidate for better thermoelectric material.     

ON QUOTIENTS OF BRAID GROUPS

ABSTRACT.

Let G be the group ?[t, t ^?1] x ?. By studying the action of the braid group B_n on the set Gⁿ , we obtain representations of B_n into a wreath product of the symmetric group and the general linear group over ?[t, t ^?1]. This in particular recovers the Burau representation of the braid group. Furthermore, some quotients of the braid group are obtained by using the representations found.