全文获取类型
收费全文 | 390篇 |
免费 | 18篇 |
国内免费 | 1篇 |
专业分类
化学 | 291篇 |
晶体学 | 2篇 |
力学 | 5篇 |
数学 | 31篇 |
物理学 | 80篇 |
出版年
2021年 | 3篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 3篇 |
2016年 | 8篇 |
2015年 | 11篇 |
2014年 | 8篇 |
2013年 | 17篇 |
2012年 | 11篇 |
2011年 | 24篇 |
2010年 | 8篇 |
2009年 | 11篇 |
2008年 | 22篇 |
2007年 | 21篇 |
2006年 | 20篇 |
2005年 | 14篇 |
2004年 | 12篇 |
2003年 | 15篇 |
2002年 | 14篇 |
2001年 | 3篇 |
2000年 | 9篇 |
1999年 | 7篇 |
1998年 | 6篇 |
1997年 | 7篇 |
1996年 | 6篇 |
1995年 | 7篇 |
1994年 | 5篇 |
1992年 | 5篇 |
1991年 | 6篇 |
1989年 | 3篇 |
1988年 | 2篇 |
1987年 | 6篇 |
1986年 | 2篇 |
1985年 | 8篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 8篇 |
1981年 | 9篇 |
1980年 | 5篇 |
1979年 | 7篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1976年 | 6篇 |
1975年 | 9篇 |
1974年 | 8篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1888年 | 2篇 |
1885年 | 2篇 |
排序方式: 共有409条查询结果,搜索用时 0 毫秒
1.
Daniela Stllner Walter Stcklein Frieder Scheller Axel Warsinke 《Analytica chimica acta》2002,470(2):3438-119
An amperometric immunosensor for hemoglobin-A1c (HbA1c) determination has been developed utilizing membrane-immobilized haptoglobin as affinity matrix fixed in front of a Pt-working electrode. The HbA1c assay was carried out in a two-step procedure including the selective hemoglobin enrichment on the sensor surface and the specific HbA1c detection by a glucose oxidase (GOx) labeled anti-HbA1c antibody. Hydrogen peroxide generated by the enzyme label was oxidized at +600 mV versus Ag/AgCl. A standard curve for HbA1c was obtained with a linear range between 0 and 25% HbA1c of total hemoglobin which correspond to 7.8–39 nM. ELISA studies confirmed the advantage of a sandwich-type format with haptoglobin as capture molecule for selective hemoglobin binding over the direct adsorption method. Results by the sandwich immunoassay showed a linear correlation within the clinically relevant range 5–20% (CV < 3). For sensor application the immobilization procedure of haptoglobin onto CDI-activated cellulose membranes was optimized. 相似文献
2.
Contrary to the results of other semi-empirical all-valence electron methods, the main features concerning the conformation of trans-benzylideneaniline are correctly accounted for using the NDDO method. Based on a modified continuum model the influence of solvents on the molecular structure was estimated. 相似文献
3.
The laccase catalyzed oxidative dimerization of salicylic esters, a rare example of a laccase-catalyzed carbon-carbon bond formation, was studied. This reaction allows the use of air as stoichiometric oxidant and proceeds in aqueous solution. The preparative scope and the mechanism of the method, which provides a new and convenient access to functionalized biaryls under mild conditions, were investigated. 相似文献
4.
Bier FF Kleinjung F Schmidt PM Scheller FW 《Analytical and bioanalytical chemistry》2002,372(2):308-313
Binding and catalytic activity of the type II restriction endonuclease EcoRI on immobilized DNA has been observed in real time using three different evanescent wave biosensors and two different immobilization techniques. The method gives direct access to the turnover number (kcat) without the necessity for the determination of any concentration or activity. The combination of different evanescent wave techniques gives access to the catalytic mechanism and allows the determination of the rate limiting step. 相似文献
5.
Frieder W. Lichtenthaler Jürgen Klotz Katsumi Nakamura 《Tetrahedron: Asymmetry》2003,14(24):3973-3986
The dimethyl esters of the 2,6-anhydro-3-deoxy-hept-2-enaric acids with
-xylo,
-lyxo,
-arabino,
-lyxo- and
-ribo-configuration were synthesized from
-galactose and
-mannose, respectively, and further characterized by their di-O-acetyl and di-O-benzoyl derivatives. Comparison of their physical data with those of Daucus carota derived products revealed (−)-daucic acid to have
-lyxo-configuration 46 rather than the previously assigned
-xylo stereochemistry 1. Dimethyl daucate 43 could be converted by acid-induced ring contraction and dehydration into naturally occurring (+)-osbeckic acid 47, thereby proving its (S)-configuration. Configurational identity in the pyranoid rings of (−)-daucic acid and KDO, together with available biosynthetic evidence on chelidonic acid 4, a leaf closing factor, suggests a joint, KDO 8-P-based pathway for their biosynthesis in plants. 相似文献
6.
Frieder Knüppel 《Journal of Geometry》1978,11(2):93-105
Let be given a geometric structure with a set of lines and a symmetric orthogonality relation. F. Bachmann defined in [3] chains of perpendiculars in such a structure, and studied those chains in Hjelmslev-groups. All AGS-groups (= groups treated in [1]) are Hjelmslev-groups, and the theory of Hjelmslev-groups includes also Hjelmslev's concept of Allgemeine Kongruenzlehre. In Hjelmslev-groups, chains of perpendiculars are closely related to the group generated by the points. We establish some results about chains of perpendiculars in special classes of Hjelmslev-groups. 相似文献
7.
Liu S Wollenberger U Halámek J Leupold E Stöcklein W Warsinke A Scheller FW 《Chemistry (Weinheim an der Bergstrasse, Germany)》2005,11(14):4239-4246
A method is provided for the recognition of glycated molecules based on their binding affinities to boronate-carrying monolayers. The affinity interaction of flavin adenine dinucleotide (FAD) and horseradish peroxidase (HRP) with phenylboronic acid monolayers on gold was investigated by using voltammetric and microgravimetric methods. Conjugates of 3-aminophenylboronic acid and 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) or 11-mercaptoundecanoic acid were prepared and self-assembled on gold surfaces to generate monolayers. FAD is bound to this modified surface and recognized by a pair of redox peaks with a formal potential of -0.433 V in a 0.1 M phosphate buffer solution, pH 6.5. Upon addition of a sugar to the buffer, the bound FAD could be replaced, indicating that the binding is reversible. Voltammetric, mass measurements, and photometric activity assays show that the HRP can also be bound to the interface. This binding is reversible, and HRP can be replaced by sorbitol or removed in acidic solution. The effects of pH, incubation time, and concentration of H(2)O(2) were studied by comparing the catalytic reduction of H(2)O(2) in the presence of the electron-donor thionine. The catalytic current of the HRP-loaded electrode was proportional to HRP concentrations in the incubation solution in the range between 5 microg mL(-1) and 0.1 mg mL(-1) with a linear slope of 3.34 microA mL mg(-1) and a correlation coefficient of 0.9945. 相似文献
8.
On the plastic deformation of bulk syndiotactic polypropylene 总被引:1,自引:0,他引:1
The plastic deformation of syndiotactic polypropylene (sPP) bulk samples has been investigated. A structural comparison of
the initial states before and after plastic deformation by necking is carried out by X-ray diffraction observations. Independent
of the initial states (amorphous, semi-crystalline with different crystal phases), only the planar all-trans crystal form of sPP is present in the deformed samples after necking. Form these results, molecular mechanisms of the plastic
deformation in the neck zone of semi-crystalline polymers will be discussed.
Received: 11 February 1997 Accepted: 15 August 1997 相似文献
9.
10.