The influence of pH on phosphatidylcholine monolayer at the air/aqueous solution interface

The measurements of the interfacial tension at the air/aqueous subphase interface as the function of pH were performed. The interfacial tension of the air–aqueous subphase interface was divided into contributions of individuals. A simple model of the influence of pH on the phosphatidylcholine monolayer at the air/hydrophobic chains of phosphatidylcholine is presented. The contributions of additive phosphatidylcholine forms (both interfacial tension values and molecular area values) depend on pH. The interfacial tension values and the molecular areas values for LH⁺, LOH⁻ forms of phosphatidylcholine were calculated. The assumed model was verified experimentally.     

HL-2M 磁体系统安装中的定位与测量

为提高磁体系统安装精度,在 HL-2M 集成大厅建立 63 个基准点构成测量基准网,并利用激光跟 踪仪等高精度测量设备建立每个磁体的局部坐标系,测量特征点的局部坐标;基于测量基准网和公共测量点,采 用最佳拟合得到坐标转换矩阵,以此得到特征点在测量基准网的位置,指导磁体安装。完成安装后的中心柱同支 撑基础的同轴度为∅2.03mm;PF1~PF4 线圈安装标高偏差为±0.5mm,与中心柱的同轴度为∅2.60mm;PF5/6/7/8 线圈与中心柱的同轴度偏差小于∅3.00mm,标高偏差在[−1mm, 1mm]区间内。基于以上方法所得到的线圈安装精 度都满足设计需求。     

Parameters characterizing acid-base equilibria between cell membrane and solution and their application to monitoring the effect of various factors on the membrane

The cell membrane is an extremely complicated object. It participates in a large number of equilibria. For this reason, it is impossible to determine the parameters of all of them. It is the purpose of this work to define a limited number of averaged parameters in order to describe the equilibria between cell membrane components and environmental components. These parameters are the total acidic functional group concentration as well as the basic group concentration and their association constants with hydrogen or hydroxyl ions. The parameters were determined using the pH dependence of the electric surface charge density. The usefulness of these parameters was checked by studying the effect of green tea on liver cells in ethanol poisoning. Ethanol provokes an increase in concentration of functional groups, positively and negatively charged, as well as an increase in the basic groups association constant and a decrease in acidic groups association constant. Administering green tea partly compensates the changes provoked by ethanol poisoning. The parameters proposed in this work, C(TA), C(TB), K(AH) and K(BOH), are suited for monitoring the changes caused by various factors.     

The Equilibria of Phosphatidylethanolamine-Cholesterol and Phosphatidylcholine–Phosphatidylethanolamine in Monolayers at the Air/Water Interface

Monolayers of phosphatidylethanolamine (PE), cholesterol (Ch), phosphatidylcholine (PC), and binary mixtures (PC-PE or PE-Ch) were investigated at the air/water interface. The surface tension values of pure and mixed monolayers were used to calculate π -A isotherms. The surface tension measurements were carried out at 20°C using an improved Teflon trough and a Nima 9000 tensiometer. The Teflon trough was filled with a subphase of triple-distilled water. Known amounts of lipid dissolved in 1-chloropropane were placed at the surface using a syringe. The interactions between phosphatidylethanolamine and cholesterol as well as phosphatidylcholine and phosphatidylethanolamine result in significant deviations from the additivity rule. An equilibrium theory to describe the behavior of monolayer components at the air/water interface was developed in order to obtain the stability constants of PC-PE and PE-Ch complexes. We considered the equilibrium between the individual components and the complex and established that phosphatidylethanolamine and cholesterol as well as phosphatidylcholine and phosphatidylethanolamine formed highly stable 1:1 complexes.     

The effect of interaction between K(+) ions and gramicidin D on the lecithin membrane interfacial tension

The effect of the presence of gramicidin D in a lecithin membrane on its interfacial tension has been studied. The studies have been carried out at various forming solution compositions and at various potassium ion concentrations in the electrolyte solution. Potassium chloride was used as the electrolyte. The complex was formed between the gramicidin molecule and K(+) ion. The following parameters describing the complex were determined: the surface area occupied by GK(+) complex (A(GK(+))), the interfacial tension of the GK(+) membrane complex (gamma(GK+)), and the stability constant of the gramicidin-K(+) complex (K). These values are 156 A(2), 1.89 mN m(-1) and 0.033 m(3) mol(-1), respectively.     

Analytical applications of electrode sensitive to labetalol in pharmaceuticals

The analytical properties of an ion-selective electrode sensitive to labetalol with a liquid membrane, based on ion-pair complexes with sodium tetraphenylborate (TPB-Na⁺) are described. The studied electrode can be used for the determination of labetalol hydrochloride as a protonated form of labetalol in pharmaceuticals. The calibration curve, e.g. EMF=f(pC _LabHCl ) is linear in the range from 10⁻⁵ to 10⁻² mol L⁻¹ with a correlation coefficient of 0.9992 and slope of 61.13 mV/decade, which is close to the Nernstian slope. The detection limit of the examined electrode is 7.20×10⁻⁶ mol L⁻¹. The influence of pH of the tested solutions on the formulation of the electrode is not as considerable since the electrode works correctly in the pH range 3.0–8.0. The main attributes of the developed electrode are: stability, good reproducibility of EMF and short response time, close to 30 seconds depending on labetalol concentration in the solution. The electrode shows good selectivity for many inorganic ions. The selectivity for drug cations is weaker due to the structural similarity of the interfering cations to labetalol. The results of labetalol determination using direct potentiometry in drugs such as Pressocard (Polpharma) and Trandate (GlaxoWellcome) were compatible with the quantity of labetalol declared by the manufacturer, and with parallel UV spectrophotometric and HPLC determinations.     

System for electrochemical studies with a four-electrode potentiostat

A four-electrode potentiostat and a system for electrochemical studies are described. The system is suitale for studies of an interface separating immiscible elecrolyte solutions by voltammetric, chronoamperometric and polarographric methods. The described systems are based on integrated operational amplifiers.     

Phase diagrams of lyotropic cholesteric fluids

Experimental investigations of lyotropic cholesterics fluids are presented which show that changes in the shape anisotropy and chirality of the micellar population determine the topology of the temperature-concentration phase diagrams. For given amounts of the substances which induce the chirality and modify the shape anisotropy of the micelles, two distinct biaxial cholesteric phases are disclosed in the phase diagrams. This is interpreted in the framework of the catastrophe theory of phase transitions.     

Effect of constant polarization on the alternating voltage generated by a vibrating interface separating immiscible electrolyte solutions in water and nitrobenzene

Measurements have been made of an alternating voltage appearing as a result of mechanical vibrations of a water-nitrobenzene interface in a capillary. It has been found that a sharp minimum appears on the plot of alternating voltage vs. polarization potential; it probably corresponds to the potential of zero charge. The measuring apparatus is presented.     

Altered membrane amino acids composition in human colorectal cancer tissue

Cancer transformation is characterized by changes in cell metabolism, which can alter the structure and function of cell membrane components, including integral membrane proteins. Qualitative and quantitative estimations of integral membrane protein are necessary for studies aimed at understanding their modifications under pathological conditions.