Formulation of Naringin Encapsulation in Zein/Caseinate Biopolymers and its Anti-adipogenic Activity in 3T3-L1 Pre-adipocytes

Journal of Cluster Science - Naringin, a dietary flavonoid of citrus fruits, was encapsulated in biodegradable proteins of zein/casein biopolymers for its enhanced functional properties. The...     

Radical scavenging activity of decalpoline,a novel compound characterized from <Emphasis Type="Italic">Decalepis hamiltonii</Emphasis>

The ethanol extract of Decalepis hamiltonii roots was subjected to antioxidant activity-guided fractionation by repeated silica gel column chromatography to get a pure antioxidant compound which was subjected to extensive analysis by UV, IR, LC-MS, and 2D HMQC NMR. The spectral data of the isolated compound was analyzed, and its structure was elucidated as decalpoline, a novel antioxidant compound not reported so far. Decalpoline exhibited multiple antioxidant properties like DPPH scavenging activity, superoxide radical scavenging activity, lipid peroxidation inhibitory activity, metal chelating and total reducing activity.     

EFFECT OF HEMATOPORPHYRIN DERIVATIVE ON HEMATOLOGICAL PARAMETERS IN RATS

Abstract— Wistar rats were injected with hematoporphyrin derivative (Hpd) intraperitoneally and kept in the dark. Rats were sacrificed 2, 24, 48 and 72 h after injection. It was observed that Hpd in the dark did not affect the hemoglobin content and number of erythrocytes, while the leukocyte count was increased and blood pH decreased. Blood levels of glucose and lactate were increased significantly. Because the food intake was similar in all the groups, glycogenolysis was suspected to be the source of increased glucose levels in blood. However, a significant increase in the glycogen content of the livers of Hpd-treated rats was observed, which rules out glycogenolysis. Hyperglycemia may result due to a number of reasons such as stimulation of the central nervous pathways innervating the liver and adrenal medulla, excessive glucogenesis in liver from glycogen and noncarbohydrate sources, emotional stress, anesthesia and hormonal effects. The present study rules out hyperglycemia due to anesthesia and glucogenesis in the liver. Maintenance of blood glucose levels is a highly complex mechanism. Further investigations to understand these mechanisms are in progress.     

EFFECTS OF HEMATOPORPHYRIN DERIVATIVE ON THE CELLULAR ENERGY METABOLISM IN THE ABSENCE AND PRESENCE OF LIGHT

Effects of Photofrin II on energy metabolism and metabolic viability were studied in a mammalian transformed cell line (BHK-21) in dark and after photo-irradiation with visible light. Cells were allowed to accumulate Photofrin by incubating for 4 h in buffer containing Photofrin (5-60 micrograms/ml). The results show that Photofrin significantly affects the cellular energy metabolism even in the absence of light; activity of cytochrome c oxidase is decreased and glucose utilization and lactate production (glycolysis) are increased. Irradiation with light resulted in a significant decrease in the activity of cytochrome c oxidase, glycolysis, ATP content, energy charge, ratios of adenine nucleotides like ATP/ADP, ATP/AMP and cell viability (dye exclusion test). Presence of inhibitors of energy metabolism, potassium cyanide (respiration) and 2-deoxyglucose (glycolysis), further enhanced the cytotoxic effects induced by hematoporphyrin derivative and light.