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Alfred Kreutzberger Elfriede Kreutzberger Hans-Joachim Saltler Hayriye Aml Aysel Gürsoy 《Journal of heterocyclic chemistry》1982,19(4):753-755
The three-component reaction comprising the interaction of 2-amino-4-antipyrinyl-5-ethylthiazole (1) with s-triazine (2) and pyrrolidine leads to 4-anitpyrinyl-5-ethyl-2-[(4-pyrrolidinyl)methyleneamino]thiazole (5). Structure 5 is supported by 1H- and 13C-NMR spectroscopy. 相似文献
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Elfriede Simon M. G. Weller Reinhard Niessner 《Analytical and bioanalytical chemistry》1998,360(7-8):824-826
The aim of the described method was the characterization of a “atrazine-mercaptopropionic acid” humic acid conjugate, which
was required for the calibration of immunoassays to determine bound residues. In order to estimate the amount of bound triazine,
an oxidative nucleophilic substitution reaction of the covalently linked “atrazine-mercaptopropionic acid” to a new triazine
derivative “atrazine-methoxyethanol” was performed. This cleavage product was quantified by gas chromatography. The conditions
for this cleavage procedure were optimized and applied to the “atrazine-mercaptopropionic acid” humic acid conjugate and to
a humic acid blank. The amount of bound “atrazine-mercaptopropionic acid” was calculated to 16.6 ± 2.5 μmol triazine per gram
humic acid, which is equivalent to 0.39 ± 0.07% atrazine.
Received: 7 August 1997 / Accepted: 12 September 1997 相似文献
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Zusammenfassung Die Sulfurierung des Thionaphthens, des 2-Methyl-, 3-Methyl-, 5-Methyl- und des 2,3-Dimethylthionaphthens, die Herstellung der entsprechenden Sulfosäurechloride,-amide und-anilide, die Ermittlung der Stellung des Sulfosäurerestes am Thionaphthenringsystem und die Wiederabspaltung der SO3H-Gruppe wird beschrieben. 相似文献
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Dr. Elfriede Dall M. Sc. Julia C. Fegg Prof. Dr. Peter Briza Prof. Dr. Hans Brandstetter 《Angewandte Chemie (International ed. in English)》2015,54(10):2917-2921
Peptide ligases expand the repertoire of genetically encoded protein architectures by synthesizing new peptide bonds, energetically driven by ATP or NTPs. Here, we report the discovery of a genuine ligase activity in human legumain (AEP) which has important roles in immunity and tumor progression that were believed to be due to its established cysteine protease activity. Defying dogma, the ligase reaction is independent of the catalytic cysteine but exploits an endogenous energy reservoir that results from the conversion of a conserved aspartate to a metastable aspartimide. Legumain’s dual protease–ligase activities are pH‐ and thus localization controlled, dominating at acidic and neutral pH, respectively. Their relevance includes reversible on–off switching of cystatin inhibitors and enzyme (in)activation, and may affect the generation of three‐dimensional MHC epitopes. The aspartate–aspartimide (succinimide) pair represents a new paradigm of coupling endergonic reactions in ATP‐scarce environments. 相似文献
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A new chiral monomer derived from cinchona alkaloid, namely O-9-(tert-butylcarbamoyl)-11-[2-(methacryloyloxy)ethylthio]-10,11-dihydroquinine 1, was employed for the preparation of enantioselective monolithic capillary columns by an in situ copolymerization with 2-hydroxyethyl methacrylate 2 (HEMA), ethylene dimethacrylate 3 (EDMA) in the presence of cyclohexanol and 1-dodecanol as porogens (UV or thermal initiation of azobisisobutyronitrile (AIBN) as radical initiator). The porous properties and the electrochromatographic behavior of the new chiral monoliths were comparatively evaluated with previously described analogs obtained from O-9-[2-(methacryloyloxy)ethylcarbamoyl]-10,11-dihydroquinidine 4 as chiral monomer. Despite close structural and physicochemical similarities of the both chiral monomers, the pore distribution profiles of the resulting monoliths were shifted typically towards larger pore diameters with the new monomer 1. Once more, it was confirmed that a low cross-linking (10 wt% related to total monomers) and a pore diameter of about 1 microm in the dry state provides the best electrochromatographic efficiency as a result of lower resistance to mass transfer (smaller C-term contribution to peak broadening) and more homogeneous flow profile (smaller A-term). Most importantly, as expected the new poly(1-co-HEMA-co-EDMA) monoliths showed enhanced enantioselectivities and in addition faster separations as compared to poly(4-co-HEMA-co-EDMA) analogs, which represents a significant improvement. Further, the elution order was reversed owing to the pseudoenantiomeric behavior of quinine- and quinidine-derived monomers. Fluorescence-labeled 9-fluorenylmethoxycarbonyl (FMOC), dansyl (DNS), 7-dimethylaminosulfonyl-1,3,2-benzoxadiazol-4-yl (DBD), carbazole-9-carbonyl (CC) amino acids could be separated with resolution values between 2 and 4 (with efficiencies typically between 100,000 and 200,000 plates/m) and fluorescence detection (variable wavelength fluorescence detector in-line with UV) yielding routinely a gain in detection sensitivities up to two orders of magnitude without specific optimization of the conditions with regards to fluorescence efficiency. 相似文献
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Willems AV Deforce DL Van den Eeckhout EG Lambert WE Van Peteghem CH De Leenheer AP Van Bocxlaer JF 《Electrophoresis》2002,23(24):4092-4103
Benzo[a]pyrene diol epoxide (BPDE) was reacted in vitro with (2'-deoxy)nucleotides and with calf thymus DNA. The modified DNA was enzymatically hydrolyzed to the 5'-monophosphate nucleotides using deoxyribonuclease I (DNA-ase I), nuclease P1 and snake venom phosphodiesterase (SVP). Most of the unmodified nucleotides were removed using solid phase extraction (SPE) in a polystyrene divinylbenzene copolymer. Three adducts could be detected and identified using capillary zone electrophoresis(negative)-ion electrospray ionization-mass spectrometry (CZE-(-)-ESI-MS) in conjunction with sample stacking. This way, not only a BPDE-dGMP adduct [(M-H)(-) at m/z 648], a well-known nucleotide adduct, could be retrieved, but also a BPDE-dAMP [(M-H)(-) at m/z 632] and a BPDE-dCMP adduct [(M-H)(-) at m/z 608] could be detected for the first time. The presence of the prominent ion at m/z 195 (the deoxyribose-phosphate ion) in the three low-energy collision-activated decomposition (CAD) spectra indicated that the adducts were formed through base-alkylation. CZE-positive ion ESI-MS/MS experiments were performed to obtain further information on base-alkylation. The absence of the loss of NH(3) from the nucleobase in each CAD spectrum points to an alkylated exocyclic NH(2) position of the nucleobase. So, the three adducts could be identified as BPDE-N(2)-dGMP, BPDE-N(6)-dAMP and BPDE-N(4)-dCMP using CZE-ESI-MS and CZE-ESI-MS/MS. 相似文献
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We consider a mathematical model of a rigid body immersed in a viscous, compressible fluid moving with a velocity prescribed on the boundary of a large channel containing the body. We assume that the Mach number is proportional to a small parameter ε and that the general boundary of the body contains small asperities of amplitude proportional to ε α for a certain α?>?0 and suppose the Navier’s slip condition on this rough boundary. We show that time averages of the drag functional converge, as ε → 0, to the corresponding time averages of the drag for the limit system, whereas the limit system is turning out to be the incompressible Navier–Stokes system with no-slip condition on the smooth limit body. 相似文献
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