排序方式: 共有2条查询结果,搜索用时 15 毫秒
1
1.
L Elfman E Heilbronn T Bartfai 《Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry》1978,32(5):370-374
We have investigated a chloroform--methanol extracted material from electric organ of Torpedo marmorata with respect to binding of [3H]-acetyl-alpha-neurotoxin and [3H]-acetylcholine. No binding was observed which is in contrast to observations with detergent extracted receptor. Further, no crossreactivity could be demonstrated between the chloroform--methanol extracted proteolipid and antibodies against the detergent extracted receptor. Labelling in the membrane with [3H]-acetyl-alpha-neurotoxin before extraction with chloroform--methanol gave a very low yield of extracted labelled compound. The protein yield is about 0.3% of the total in the homogenate and only 0.04% of the specifically bound neurotoxin. Using the alkylating affinity label [3H]-N-propylbenzilylcholine mustard (1) and also [3H]-atropine, which both bind, at low concentrations, specifically to the muscarinic acetylcholine receptor, binding was studied to the chloroform--methanol extracted proteolipid from rat brain cortex. No specific binding could, however, be demonstrated. Prelabelling in the membrane with 1 and subsequent extraction with chloroform--methanol gave a protein yield of about 1% and an extraction of 26% of the specifically bound label. 相似文献
2.
Nilsson E. J. Charlotta Kristiansson Per Ros Linus De La Rosa Nathaly Elfman Mikael Hålenius Ulf Pallon Jan Skogby Henrik 《Journal of Radioanalytical and Nuclear Chemistry》2017,311(1):355-364
Journal of Radioanalytical and Nuclear Chemistry - Ion beam analysis has for decades been used as a tool for geochemical analysis of trace elements using both X-rays (particle induced X-ray... 相似文献
1