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Eiamwat Jirawat Loha Veara Prokop Aleš Tanner Robert D. 《Applied biochemistry and biotechnology》1998,(1):559-567
The aqueous protein solution from kudzu(Pueraria lobata) vine retting broth, without the addition of other surfactants, was foam-fractionated in a vertical tubular column with multiple
sampling ports. Time-varying trajectories of the total protein levels were determined to describe the protein behavior at
six positions along the 1-m column. The lowest two trajectories of this batch process represented a loss of proteins from
the bulk liquid and tended to merge and decay together in time; the other trajectories displayed a gain in proteins in the
foam phase. These upper column port protein concentration trajectories generally increased in time up to 45 min, followed
by a decrease, reflecting the removal of proteins from the column ports. The foam became dryer as it passed up the column
to the top port. The protein concentration was about 5–8×higher in the top port foam than in the initial bulk solution, mainly
as a result of liquid drainage from the foam along the column axis. This concentration increase in the collected foam was
dependent on the initial pH of the bulk solution. The mol-wt profile of the proteins in the concentrated foam effluent was
determined by one-dimensional gel electrophoresis. An analysis of the gel electropherograms indicated that the most abundant
proteins could be cellulases and pectinases. 相似文献
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