Specificity of the lipase from cottonseed

Conclusions 1. The lipase from cotton seeds possesses lipase, esterase, and tweenase activities.2. The broad substrate specificity of the lipase is connected with its capacity for functioning both in homogeneous and in heterogeneous media. A mechanism of the action of the lipase explaining the specificity of its action is proposed.Institute of the Chemistry of Plant Substances, Academy of Sciences of the UzbekSSR. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 602–607, September–October, 1970.     

Lipolytic activities of enzymes in cotton seeds

Chemistry of Natural Compounds -     

Lipolytic enzymes of cotton seeds V. Triacetinase

Summary A triacetinase has been isolated from cotton seeds by the methods of gel filtration and ion-exchange chromatography. The homogeneity of the enzyme was shown by rechromatography, electrophoresis, ultracentrifugation, and a determination of the terminal amino acid. The molecular weight of the triacetinase obtained by four different methods (column gel filtration, thin-layer chromatography, ultracentrifugation, and amino-acid composition) is 18,000±1200. The amino-acid composition and the N-terminal amino-acid residue — methionine — have determined.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 767–770, November–December, 1974.