Inactivation Activities of Ozonated Water,Slightly Acidic Electrolyzed Water and Ethanol against SARS-CoV-2

This study aimed to compare the SARS-CoV-2-inactivation activity and virucidal mechanisms of ozonated water (OW) with those of slightly acidic electrolyzed water (SAEW) and 70% ethanol (EtOH). SARS-CoV-2-inactivation activity was evaluated in a virus solution containing 1%, 20% or 40% fetal bovine serum (FBS) with OW, SAEW or EtOH at a virus-to-test solution ratio of 1:9, 1:19 or 1:99 for a reaction time of 20 s. EtOH showed the strongest virucidal activity, followed by SAEW and OW. Even though EtOH potently inactivated the virus despite the 40% FBS concentration, virus inactivation by OW and SAEW decreased in proportion to the increase in FBS concentration. Nevertheless, OW and SAEW showed potent virucidal activity with 40% FBS at a virus-to-test solution ratio of 1:99. Real-time PCR targeting the viral genome revealed that cycle threshold values in the OW and SAEW groups were significantly higher than those in the control group, suggesting that OW and SAEW disrupted the viral genome. Western blotting analysis targeting the recombinant viral spike protein S1 subunit showed a change in the specific band into a ladder upon treatment with OW and SAEW. OW and SAEW may cause conformational changes in the S1 subunit of the SARS-CoV-2 spike protein.     

Study of alkaloids of the Siberian and Altai flora. 7. Synthesis of 18-amino-18-deoxy derivatives of elatidine

Reduction of elatidal oxime and imines based on methylamine, ethanolamine, tyramine, and S- and (±)-alaninols gave rise to the 18-amino-18-deoxy derivatives of elatidine.     

Arylnaphthalene lignans ofHaplophyllum dauricum. The structure of daurinol

The arylnaphthalene lignans justicidin B and daurinol I have been isolated from the epigeal part ofHaplophyllum dauricum (L.) G. Don.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 295–297, May–June, 1981.     

Chemical constituents isolated from Zygophyllum melongena Bunge growing in Mongolia

We report the first investigation of the chemical constituents of Zygophyllum melongena Bunge, a species growing in Mongolia. The quinovic acid glycosides 3-O-(β-D-glucopyranosyl)quinovic acid and 3-O-(β-D-glucopyranosyl)quinovic acid (28→1)-(β-D-glucopyranosyl) ester were identified in the chloroform fraction along with the flavonoid glycoside astragalin. The n-butanol fraction contained (+)-D-pinitol as the major component, a cyclitol with anti-diabetic properties. The structures of the isolated natural products were confirmed using ESI-MS and NMR spectroscopy (¹H, ¹³C, COSY, HSQC, HMBC, NOESY and ROESY). This is the first report of the isolation of (+)-D-pinitol from the genus Zygophyllum.     

Components of Artemisia santolinifolia

Institute of Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 697–698, September–October, 1990.     

Arylnaphthalene lignans ofHaplophyllum dauricum. The structure of daurinol

The arylnaphthalene lignans justicidin B and daurinol I have been isolated from the epigeal part ofHaplophyllum dauricum (L.) G. Don.     

Alkaloids of Oxytropis. I

From the epigeal parts of two species ofOxytropis growing in Mongolia we have isolated the new alkaloids (+)-N-benzoyl-2-hydroxy-2-phenylethylamine and (–)-N-nicotinoyl-2-hydroxy-2-phenylethylamine, and also N-benzoyl-2-phenylethylamine, this being the first time this substance has been found in nature. The structures of the alkaloids were established on the basis of a comparative analysis of their spectral characteristics. Features of the mass-spectrometric behavior of this compound have been studied.Institute of Chemistry, Academy of Sciences of the Mongolian Peoples' Republic. Institute of Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, Nos. 3,4, pp. 388–393, May–August, 1992.     

Alkaloids of Aconitum barbatum. Structure of bataconine

The epigeal part ofAconitum barbatum gathered in the early vegetation period in the environs of Ulan-Bator has yielded delcosine, lycoctonine, songorine, and the new base bataconine. The structure of bataconine has been shown on the basis of spectral characteristics (IR, NMR, and mass spectra) and also of a correlation with the known alkaloid neoline.Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 237–239, March–April, 1988.