排序方式: 共有18条查询结果,搜索用时 31 毫秒
1.
Elsayim Rasha AlOthman Monerah Alkhulaifi Manal Ali Rehab Doud Mohammed Elnagar Doaa 《Molecules (Basel, Switzerland)》2021,26(7)
Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells. 相似文献
2.
Lupoli TJ Tsukamoto H Doud EH Wang TS Walker S Kahne D 《Journal of the American Chemical Society》2011,133(28):10748-10751
The β-lactams are the most important class of antibiotics in clinical use. Their lethal targets are the transpeptidase domains of penicillin binding proteins (PBPs), which catalyze the cross-linking of bacterial peptidoglycan (PG) during cell wall synthesis. The transpeptidation reaction occurs in two steps, the first being formation of a covalent enzyme intermediate and the second involving attack of an amine on this intermediate. Here we use defined PG substrates to dissect the individual steps catalyzed by a purified E. coli transpeptidase. We demonstrate that this transpeptidase accepts a set of structurally diverse D-amino acid substrates and incorporates them into PG fragments. These results provide new information on donor and acceptor requirements as well as a mechanistic basis for previous observations that noncanonical D-amino acids can be introduced into the bacterial cell wall. 相似文献
3.
EE Jung M Kalontarov DF Doud MD Ooms LT Angenent D Sinton D Erickson 《Lab on a chip》2012,12(19):3740-3745
Microalgae are a promising feedstock for sustainable biofuel production. At present, however, there are a number of challenges that limit the economic viability of the process. Two of the major challenges are the non-uniform distribution of light in photobioreactors and the inefficiencies associated with traditional biomass processing. To address the latter limitation, a number of studies have demonstrated organisms that directly secrete fuels without requiring organism harvesting. In this paper, we demonstrate a novel optofluidic photobioreactor that can help address the light distribution challenge while being compatible with these chemical secreting organisms. Our approach is based on light delivery to surface bound photosynthetic organisms through the evanescent field of an optically excited slab waveguide. In addition to characterizing organism growth-rates in the system, we also show here, for the first time, that the photon usage efficiency of evanescent field illumination is comparable to the direct illumination used in traditional photobioreactors. We also show that the stackable nature of the slab waveguide approach could yield a 12-fold improvement in the volumetric productivity. 相似文献
4.
Darrin Doud 《manuscripta mathematica》1998,95(1):463-469
We present an algorithm which uses the analytic parameterization of elliptic curves to rapidly calculate torsion subgroups,
and calculate its running time. This algorithm is much faster than the “traditional” Lutz-Nagell algorithm used by most computer
algebra systems to calculate torsion subgroups.
This article was processed by the author using the TEX style filePjour1g from Springer-Verlag 相似文献
5.
6.
Angie?S?Mah Andrew?EH?Elia Geeta?Devgan Jason?Ptacek Mike?Schutkowski Michael?Snyder Michael?B?Yaffe Raymond?J?DeshaiesEmail author 《BMC biochemistry》2005,6(1):22
Background
The mitotic exit network (MEN) is a group of proteins that form a signaling cascade that is essential for cells to exit mitosis in Saccharomyces cerevisiae. The MEN has also been implicated in playing a role in cytokinesis. Two components of this signaling pathway are the protein kinase Dbf2 and its binding partner essential for its kinase activity, Mob1. The components of MEN that act upstream of Dbf2-Mob1 have been characterized, but physiological substrates for Dbf2-Mob1 have yet to be identified. 相似文献7.
We study a canonical basis for spaces of weakly holomorphic modular forms of weights 12, 16, 18, 20, 22, and 26 on the full
modular group. We prove a relation between the Fourier coefficients of modular forms in this canonical basis and a generalized
Ramanujan τ-function, and use this to prove that these Fourier coefficients are often highly divisible by 2. 相似文献
8.
Doud EH Perlstein DL Wolpert M Cane DE Walker S 《Journal of the American Chemical Society》2011,133(5):1270-1273
The reactions of two bacterial TIM barrel prenyltransferases (PTs), MoeO5 and PcrB, were explored. MoeO5, the enzyme responsible for the first step in moenomycin biosynthesis, catalyzes the transfer of farnesyl to 3-phosphoglyceric acid (3PG) to give a product containing a cis-allylic double bond. We show that this reaction involves isomerization to a nerolidyl pyrophosphate intermediate followed by bond rotation prior to attack by the nucleophile. This mechanism is unprecedented for a prenyltransferase that catalyzes an intermolecular coupling. We also show that PcrB transfers geranyl and geranylgeranyl groups to glycerol-1-phosphate (G1P), making it the first known bacterial enzyme to use G1P as a substrate. Unlike MoeO5, PcrB catalyzes prenyl transfer without isomerization to give products that retain the trans-allylic bond of the prenyl donors. The TIM barrel family of PTs is unique in including enzymes that catalyze prenyl transfer by distinctly different reaction mechanisms. 相似文献
9.
Maria Doudékova-Puydebois 《Monatshefte für Mathematik》2002,135(1):11-24
In this paper, we investigate the class of numeration systems and we study the associated dynamical systems, called odometers. It is shown that these odometers are measure-theoretically
isomorphic to rank one transformations on the unit interval, constructed by a cutting-stacking method. Furthermore, a symbolic
coding leads to isomorphic shift systems arising from substitutions. Some skew products of the odometers by cocycles related
to the sum of digits are shown to be ergodic.
Received 5 March 2001; in revised form 16 August 2001 相似文献
10.
Maria Doudékova-Puydebois 《Monatshefte für Mathematik》2002,30(3):11-24
In this paper, we investigate the class of numeration systems and we study the associated dynamical systems, called odometers. It is shown that these odometers are measure-theoretically isomorphic to rank one transformations on the unit interval, constructed by a cutting-stacking method. Furthermore, a symbolic coding leads to isomorphic shift systems arising from substitutions. Some skew products of the odometers by cocycles related to the sum of digits are shown to be ergodic. 相似文献