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1.
DETECTION OF DNA-PSORALEN PHOTOADDUCTS in situ 总被引:1,自引:0,他引:1
ZOFIA ZAREBSKA† MARIA JARZABEK-CHORZELSKA ‡ GENOWEFA RZSA WIESLAW GLISKI MARIA PAWIKA TADEUSZ CHORZELSKI STEFANIA JABLOKA 《Photochemistry and photobiology》1984,39(3):307-312
Abstract— An immunological method, with the use of specific immune serum, has been developed for detection of 8-methoxypsoralen (8-MOP) photoadducts to DNA, formed in situ in cell nuclei, after combined treatment with 8MOP and UV-A irradiation (Zarçbska et al. , 1978). Lymphocytes fixed on slides or in suspension, and cryostat sections of different mammalian tissues, served as antigenic substrate, after treatment with 8-MOP and UV-A in vitro. Specific fluorescence in these substrates was detected in the nuclei after treatment with 30 ˜ 140 kJ/m2 UV-A in the presence of 0.1-0.3 μg/cm2 8-MOP. PHA-stimulated-lymphocytes appeared to be the most sensitive substrate.
However, hairless mice treated with high doses of UV-A in vivo , 70 ˜ 360 kJ/m2 did not reveal a specific fluorescence of epidermal nuclei, unless a high local concentration of 8-MOP was attained.
The apparent discrepancy in the level of photoadduct detection between the in vitro and in vivo treated specimens was explained by the low number of DNA-8-MOP-photoadducts formed in vivo under these experimental conditions. The relevance of these findings to the role of DNA-8-MOP-photoadducts formed during PUVA photochemotherapy is discussed. 相似文献
However, hairless mice treated with high doses of UV-A in vivo , 70 ˜ 360 kJ/m
The apparent discrepancy in the level of photoadduct detection between the in vitro and in vivo treated specimens was explained by the low number of DNA-8-MOP-photoadducts formed in vivo under these experimental conditions. The relevance of these findings to the role of DNA-8-MOP-photoadducts formed during PUVA photochemotherapy is discussed. 相似文献
2.
Walter J. Deal 《International journal of quantum chemistry》1989,35(4):513-518
The local energy is examined as an indicator of the accuracy of approximate wave functions for the ground state of helium. It is observed that at a given point (1) an inaccurate local energy may or may not correspond to an inaccurate value of the wave function or probability density, but (2) a value of the local energy within 0.1 a.u. of the ground-state energy corresponds to a value of the approximate wave function or probability density within about 10% of that for the ground-state wave function. 相似文献
3.
4.
S. B. Deal 《Fresenius' Journal of Analytical Chemistry》1955,147(4):320
Ohne Zusammenfassung 相似文献
5.
ABSTRACT. Let G be the group ?[t, t ?1] x ?. By studying the action of the braid group Bn on the set Gn , we obtain representations of Bn into a wreath product of the symmetric group and the general linear group over ?[t, t ?1]. This in particular recovers the Burau representation of the braid group. Furthermore, some quotients of the braid group are obtained by using the representations found. 相似文献
6.
Raman and luminescence spectroscopy were used to determine the structure of alkali borate tungstate glasses: M2O(B2O3)2·xWO3, M = Li or Na (0 < x < 1). Raman scattering results showed the dominant tungstate species in these photochromic glasses to be tetrahedral WO4=. At high concentrations of WO3, WO3·H2O, and W2O7= are also present. Luminescence measurements provided evidence for an octahedral WO3 structure not identified by the Raman results. The results also revealed a possible change in the structure of the glasses similar to that observed in alkali borate glasses and associated with the “borate anomaly”. In addition, preliminary measurements are reported on the variation of the band gap, density, index of refraction, and the elastic coefficient C11 determined by Brillouin scattering with composition. 相似文献
7.
This paper reviews some of the recent efforts in the area of microwave–optical interactions applied to active integrated antennas and arrays. Two optical–microwave techniques and examples of their application are presented. First, antenna remoting via optical means is discussed. Second, optical control of microwave devices in active antenna circuits is demonstrated. 相似文献
8.
The phase equilibria in the Ga-As-Cr system were determined for As ? 50 a/o from room temperature to 1300°C by calorimetric and x-ray examination of appropriately prepared samples. Several corrections were made in the literature values for properties of Cr-As binary compounds. The ternary diagram shows that GaAs is not a dominant compound in the system and GaAs and Cr cannot coexist under equilibrium conditions. From one ternary tie triangle region and a solid plus liquid region, appropriate solubility and diffusion sources for Cr in GaAs for most temperatures can be obtained. 相似文献
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10.
The evaluation of interatomic interactions at large separations (R) typically involves neglecting electron exchange, treating the Coulomb interaction between atoms as a perturbation, neglecting third- and higher-order energy contributions, and approximating the Coulomb interaction by a short expansion in spherical harmonics and, usually, powers of R?1. This last approximation, using an approximate perturbing Hamiltonian to evaluate a second-order perturbed energy, is examined here; error bounds and a simple correction are introduced. Three illustrative applications to the H? H+ interaction are given: the error incurred by truncating the spherical-harmonic expansion is bounded, the R?1 expansion is corrected for the overlap of the “atomic” charge distributions, and the R?1 expansion is analyzed to see why it works as well as it does. 相似文献