排序方式: 共有26条查询结果,搜索用时 15 毫秒
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Ryan Guterman Bradley M. Berven T. Chris Corkery Heng‐Yong Nie Mike Idacavage Elizabeth R. Gillies Paul J. Ragogna 《Journal of polymer science. Part A, Polymer chemistry》2013,51(13):2782-2792
An array of highly fluorinated polymerizable phosphonium salts (HFPPS) were synthesized from PH3 and utilized in UV‐curable formulations. Inclusion of these salts at very low loading (0.1–1 wt %) into hexanediol diacrylate (HDDA) resulted in hydrophobic surfaces. The water repellency was achieved with short C4F9 fluorocarbon appendages in the monomer as opposed to the bioaccumulative C8F17 appended polymers. The physical properties of these new monomers were also characterized. The molecular architecture of the monomers had a pronounced effect on both their physical properties along with the degree of hydrophobicity imparted in the polymer. Salts utilizing the bis(trifluoromethylsulfonyl)imide anion displayed excellent compatibility with HDDA, while the chloride salts were insoluble. Time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) confirmed the presence of the HFPPS at the surface of the polymer coating. For the first time this demonstrates how these salts may be used to functionalize the surface of a UV‐cured film with ionic species. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013, 51, 2782–2792 相似文献
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Ultrafast and selective labeling of endogenous proteins using affinity-based benzotriazole chemistry
Xiaoyi Xin Yu Zhang Massimiliano Gaetani Susanna L. Lundstrm Roman A. Zubarev Yuan Zhou Dale P. Corkery Yao-Wen Wu 《Chemical science》2022,13(24):7240
Chemical modification of proteins is enormously useful for characterizing protein function in complex biological systems and for drug development. Selective labeling of native or endogenous proteins is challenging owing to the existence of distinct functional groups in proteins and in living systems. Chemistry for rapid and selective labeling of proteins remains in high demand. Here we have developed novel affinity labeling probes using benzotriazole (BTA) chemistry. We showed that affinity-based BTA probes selectively and covalently label a lysine residue in the vicinity of the ligand binding site of a target protein with a reaction half-time of 28 s. The reaction rate constant is comparable to the fastest biorthogonal chemistry. This approach was used to selectively label different cytosolic and membrane proteins in vitro and in live cells. BTA chemistry could be widely useful for labeling of native/endogenous proteins, target identification and development of covalent inhibitors.Affinity-based benzotriazole (BTA) probes selectively and covalently label native proteins or endogenous proteins in cells with a fast reaction rate. It is enormously useful for characterizing protein function in biological systems and for drug development. 相似文献
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Corkery LJ Pang H Schneider BB Covey TR Siu KW 《Journal of the American Society for Mass Spectrometry》2005,16(3):363-369
An automated nanospray system based on chip technology (the NanoMate) was successfully interfaced to a modified Particle Discriminator Interface on a triple quadrupole mass spectrometer. A number of the interface parameters were optimized to improve the sampling efficiency for ions from the chip-based system. Analytical performance was assessed using a number of biochemicals as well as via a methodology for a pharmaceutical that passed validation as required by Good Laboratory Practices. Infusion analyses in flow rates <1 microL/min provided advantages in terms of throughput and sample consumption when compared to other methodologies based on liquid chromatography. 相似文献
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Dr. Daniel J. Foley M. Sc. Sarah Zinken Dr. Dale Corkery Assist. Prof. Luca Laraia Dr. Axel Pahl Prof. Dr. Yao-Wen Wu Prof. Dr. Herbert Waldmann 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(30):12570-12576
Pseudo-natural-product (NP) design combines natural product fragments to provide unprecedented NP-inspired compounds not accessible by biosynthesis, but endowed with biological relevance. Since the bioactivity of pseudo-NPs may be unprecedented or unexpected, they are best evaluated in target agnostic cell-based assays monitoring entire cellular programs or complex phenotypes. Here, the Cinchona alkaloid scaffold was merged with the indole ring system to synthesize indocinchona alkaloids by Pd-catalyzed annulation. Exploration of indocinchona alkaloid bioactivities in phenotypic assays revealed a novel class of azaindole-containing autophagy inhibitors, the azaquindoles. Subsequent characterization of the most potent compound, azaquindole-1, in the morphological cell painting assay, guided target identification efforts. In contrast to the parent Cinchona alkaloids, azaquindoles selectively inhibit starvation- and rapamycin-induced autophagy by targeting the lipid kinase VPS34. 相似文献
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Luca Laraia Guillaume Garivet Daniel J. Foley Nadine Kaiser Sebastian Müller Sarah Zinken Thomas Pinkert Julian Wilke Dale Corkery Axel Pahl Sonja Sievers Petra Janning Christoph Arenz Yaowen Wu Raphaël Rodriguez Herbert Waldmann 《Angewandte Chemie (International ed. in English)》2020,59(14):5721-5729
Chemical proteomics is widely applied in small‐molecule target identification. However, in general it does not identify non‐protein small‐molecule targets, and thus, alternative methods for target identification are in high demand. We report the discovery of the autophagy inhibitor autoquin and the identification of its molecular mode of action using image‐based morphological profiling in the cell painting assay. A compound‐induced fingerprint representing changes in 579 cellular parameters revealed that autoquin accumulates in lysosomes and inhibits their fusion with autophagosomes. In addition, autoquin sequesters Fe2+ in lysosomes, resulting in an increase of lysosomal reactive oxygen species and ultimately cell death. Such a mechanism of action would have been challenging to unravel by current methods. This work demonstrates the potential of the cell painting assay to deconvolute modes of action of small molecules, warranting wider application in chemical biology. 相似文献
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Sörensen MH Valle-Delgado JJ Corkery RW Rutland MW Alberius PC 《Langmuir : the ACS journal of surfaces and colloids》2008,24(13):7024-7030
We introduced the novel technique of AFM-porosimetry and applied it to measure the total pore volume of porous particles with a spherical geometry. The methodology is based on using an atomic force microscope as a balance to measure masses of individual particles. Several particles within the same batch were measured, and by plotting particle mass versus particle volume, the bulk density of the sample can be extracted from the slope of the linear fit. The pore volume is then calculated from the densities of the bulk and matrix materials, respectively. In contrast to nitrogen sorption and mercury porosimetry, this method is capable of measuring the total pore volume regardless of pore size distribution and pore connectivity. In this study, three porous samples were investigated by AFM-porosimetry: one ordered mesoporous sample and two disordered foam structures. All samples were based on a matrix of amorphous silica templated by a block copolymer, Pluronic F127, swollen to various degrees with poly(propylene glycol). In addition, the density of silica spheres without a template was measured by two independent techniques: AFM and the Archimedes principle. 相似文献
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Combined emulsion and solvent evaporation (ESE) synthesis route to well-ordered mesoporous materials
Andersson N Kronberg B Corkery R Alberius P 《Langmuir : the ACS journal of surfaces and colloids》2007,23(3):1459-1464
Control over morphology and internal mesostructure of surfactant templated silicas remains a challenge, especially when considering scaling laboratory syntheses up to industrial volumes. Here we report a method combining emulsification with the evaporation-induced self-assembly (EISA) method for preparing spherical, mesoporous silica particles. This emulsion and solvent evaporation (ESE) method has several potential advantages over classic precipitation routes: it is easily scaled while providing superior control over stoichiometric homogeneity of templating surfactants and inorganic precursors, and particle sizes and distributions are determined by principles developed for manipulating droplet sizes within water-in-oil emulsions. To demonstrate the method, triblock copolymer P104 is used as a templating amphiphile, generating unusually well-ordered 2D hexagonal (P6mm) mesoporous silica, while particle sizes and morphologies were controlled by varying the type of emulsifier and the method for emulsification. 相似文献