7.
Layers of recombinant spider silks and native silks from silk worms were prepared by spin-coating and casting of various solutions.
FT-IR spectra were recorded to investigate the influence of the different mechanical stress occurring during the preparation
of the silk layers. The solubility of the recombinant spider silk proteins SO1-ELP, C16, AQ24NR3, and of the silk fibroin
from
Bombyx mori were investigated in hexafluorisopropanol, ionic liquids and concentrated salt solutions. The morphology and thickness of
the layers were determined by Atomic Force Microscopy (AFM) or with a profilometer. The mechanical behaviour was investigated
by acoustic impedance analysis by using a quartz crystal microbalance (QCMB) as well as by microindentation.
The density of silk layers (d<300 nm) was determined based on AFM and QCMB measurements. At silk layers thicker than 300 nm
significant changes of the half-band-half width can be correlated with increasing energy dissipation. Microhardness measurements
demonstrate that recombinant spider silk and sericine-free
Bombyx mori silk layers achieve higher elastic penetration modules E
EP and Martens hardness values H
M than those of polyethylenterephthalate (PET) and polyetherimide (PEI) foils.
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