Synthesis of new 2-vinylation products of indole via a Michael-type addition reaction with dimethyl acetylenedicarboxylate and their Diels-Alder reactivity as precursors of new carbazoles

Reaction of 4,7-dihydroindole and dimethyl acetylenedicarboxylate provided a convenient route to functionalized 2-vinylindoles. Diels-Alder reactions of the 2-vinylindoles with naphthoquinone, p-benzoquinone, 1,2-dicyano-4,5-dichloroquinone, N-phenyltriazolinedione, and tetracyanoethylene were investigated to give [c]annelated 1,2-dihydro, 1,2,3,4-tetrahydro, and fully aromatized carbazoles. The structure and formation mechanism of both 2-vinylindoles and their cycloadduct are discussed.     

Ring opening of epoxides with NaHSO4: isolation of β-hydroxy sulfate esters and an effective synthesis for trans-diols

Sodium hydrogen sulfate (NaHSO₄) was observed to be highly effective as a reagent or catalyst in the ring-opening reactions of epoxides under mild conditions. Reaction of epoxides with NaHSO₄ gave isolable β-hydroxy sulfate esters and vicinal diols. Experimenting with different epoxides, the study investigated the scope of the ring-opening reaction.     

A comparative study between LMS and PSO algorithms on the optical channel estimation for radio over fiber systems

In this paper the particle swarm optimization (PSO) and least mean square (LMS) algorithms are comparatively studied to estimate the optical communication channel parameters for radio over fiber systems. It is observed that especially in low noise one tap optical channels, the convergence of LMS algorithm is approximately same with PSO algorithm. On the other hand, as a communication medium, selecting high noisy fiber optical channels or free space optical channels; PSO reaches better mean square error values. The computational complexity which is one of the most important features for optimization algorithms has also been taken into account.     

Investigation of temperature dependent electrical and impedance characteristics of bulk Zn0.95Co0.05O

Journal of Sol-Gel Science and Technology - Zn0.95Co0.05O was synthesized by sol-gel method and then Zn0.95Co0.05O pellet was prepared to investigate its structural, morphological, electrical, and...     

Electrochemical probe DNA design in PCR amplicon sequence for the optimum detection of microbiological diseases

Direct electrochemical genosensor was developed for the detection of a probe sequence relative position in a PCR amplicon for the optimum detection of bacterial and microbiological diseases, in this study. The genosensor relies on a label-free electrochemical detection. The amino-linked inosine modified (guanine-free) coequal capture probes which were chosen from different parts of a PCR amplicon, immobilized on to disposable pencil graphite electrodes (PGE) by electrostatically and covalently. As a model case Hepatitis B virus (HBV) genome amplicon was used for the detection and specification. Hybridization was occurred after surface coverage with denatured amplicons. After hybridization, optimum probe sequence position was identified by using the differences between the responses of guanine oxidation signals. The results of this study might have a great convenience for the microbiological diseases detection applications such as DNA micro arrays.     

Nonlinear degenerate parabolic equations with time dependent singular coefficients

We are concerned with the nonexistence of positive solutions of the nonlinear parabolic partial differential equations in a cylinder Ω × (0, T) with initial condition u(., 0) = u₀(.) ? 0 and vanishing on the boundary ?Ω × (0, T), given by where $\Omega \in \mathbf {R}^NWe are concerned with the nonexistence of positive solutions of the nonlinear parabolic partial differential equations in a cylinder Ω × (0, T) with initial condition u(., 0) = u₀(.) ? 0 and vanishing on the boundary ?Ω × (0, T), given by where $\Omega \in \mathbf {R}^N$ (resp. a Carnot Carathéodory metric ball in $\mathbf {R}^{2N+1})$ with smooth boundary and the time dependent singular potential function V(x, t) ∈ L¹_loc(Ω × (0, T)), $\alpha , \beta \in \mathbf {R}$, 1 < p < N, p ? 1 + α + β > 0. We find the best lower bounds for p + β and provide proofs for the nonexistence of positive solutions. © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim     

Electrochemical Genoassay Design for Allele‐Specific Detection of Toll‐Like Receptor‐2 Gene Polymorphism

An allele‐specific voltammetric genoassay for the detection of allele‐specific toll‐like receptor‐2 gene arg753gln polymorphism (TLR‐2) from polymerase chain reaction (PCR) amplified real samples was described in this study. Meldola blue (MDB), an intercalator molecule, was used as hybridization label. The wild‐type and mutant type oligonucleotide probes were immobilized onto disposable graphite electrode surfaces by covalent attachment method. The extent of hybridization between probe and target sequences was determined by using differential pulse voltammetry (DPV). As a result of the interaction between MDB and DNA at electrode surface, the MDB signal observed from probe sequence before hybridization and after hybridization with MM sequence is lower than that observed after hybridization with complementary sequence. The differences between the MDB reduction peaks obtained from probe modified, hybrid modified and MM modified electrode were used to detect TLR‐2 from PCR amplified real samples. The discrimination of homozygous and heterozygous alleles was also established by comparing the peak currents of MDB reduction signals. Numerous factors affecting the target hybridization and indicator binding reactions are optimized to maximize the sensitivity.