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1.
KL Britton HF Rogers Y Asano T Dairi Y Kato TJ Stillman DW Rice 《Acta crystallographica. Section D, Biological crystallography》1998,53(4):124-126
The novel NAD+-linked opine dehydrogenase from a soil isolate Arthrobacter sp. strain 1C belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificites. Crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 ? resolution. X-ray precession photographs have established that the crystals belong to space group P21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 ? and a single subunit in the asymmetric unit. The elucidation of the three-dimensional structure of this enzyme will provide a structural framework for this novel class of dehydrogenases to enable a comparison to be made with other enzyme families and also as the basis for mutagenesis experiments directed towards the production of natural and synthetic opine-type compounds containing two chiral centres. 相似文献
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Croom B.P. Burden D. Jin H. Vonk N.H. Hoefnagels J.P.M. Smaniotto B. Hild F. Quintana E. Sun Q. Nie X. Li X. 《Experimental Mechanics》2021,61(2):395-410
Experimental Mechanics - The quality of Digital Volume Correlation (DVC) full-field displacement measurements depends directly on the characteristics of the X-ray Computed Tomography (XCT)... 相似文献
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L. Ke R.S. Kumar S.J. Chua A.P. Burden 《Applied Physics A: Materials Science & Processing》2005,81(5):969-974
Indium tin oxide (ITO) coated polycarbonate (PC) multilayer flexible substrates have been subjected to different cycles of bending. Atomic force microscopy (AFM) results on bent ITO surfaces show that bending results in much rougher ITO surfaces. The Ca degradation test shows that the ITO cracks are perpendicular to the flexing direction and that barrier performance deteriorated after bending. When an organic light emitting device is fabricated on the bent substrate, electrical and optical performance decrease. This can be attributed to moisture and oxygen permeated through deteriorated substrate and barrier degraded polymer and oxidized cathode materials. However, optical microscopy observation found that the dark spots have less relationship with the size and position of the cracks on oxide film. Instead to a great extent, they are directly related to the ITO surface spikes. The results further prove that the dark spot is due to electrical stress generated by intense local current at sharp anode and cathode points, which degrade the polymer causing the formation of the dark center. PACS 78.66.Qn; 82.35.Cd; 62.60.Mk 相似文献
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The quadrupole hyperfine structure in the rotational spectrum of two isotopic forms of imidazole has been analyzed to obtain principal nuclear quadrupole coupling constants for the two nonequivalent nitrogen atoms. The results have been compared with field gradient tensors calculated using INDO molecular orbital wavefunctions and some implications for the electronic structure of imidazole are mentioned. Comparisons with pyrazole and the NQR results for imidazole are also made. 相似文献
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We estimate the distribution of the eigenvalues of the discrete Laplacian on a bounded set inR
n
. The proof is based on a variational technique similar to that used by Weyl for the Laplacian. As an application of our estimates we prove stability in the maximum norm for the Crank-Nicolson method for the heat equation on a bounded set.The research of the second author was supported in part by National Science Foundation grant GP-30735X. 相似文献