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Annie Grouiller Bolenketa Nonga Marie-Line Navarro Patrick Molière Henri Pacheco 《Journal of carbohydrate chemistry》2013,32(3):507-524
Abstract Various 4-deoxy-α or β-erythro or threo-pentopyranoses aminated on position 1 [6 and 19]. 2 [38] or 3 [9, 22, 23, 24, 32 and 35] were synthesised from 2-methoxy-5,6-dihydro-2H-pyran [1] and methyl 2,3-anhydro-4-deoxy-α and β-DL-erythro-pentopyranoside [11]. Different reactions were investigated including azidation. cis-oxyamination, epimine formation and oxirane aminolysis. All these amino sugars were converted into their chloroethylureido derivatives. 相似文献
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Olivier E. Nonga Darja Lavogina Erki Enkvist Katrin Kestav Apirat Chaikuad Sarah E. Dixon-Clarke Alex N. Bullock Sergei Kopanchuk Taavi Ivan Ramesh Ekambaram Kaido Viht Stefan Knapp Asko Uri 《Molecules (Basel, Switzerland)》2021,26(14)
We performed an X-ray crystallographic study of complexes of protein kinase PIM-1 with three inhibitors comprising an adenosine mimetic moiety, a linker, and a peptide-mimetic (d-Arg)6 fragment. Guided by the structural models, simplified chemical structures with a reduced number of polar groups and chiral centers were designed. The developed inhibitors retained low-nanomolar potency and possessed remarkable selectivity toward the PIM kinases. The new inhibitors were derivatized with biotin or fluorescent dye Cy5 and then applied for the detection of PIM kinases in biochemical solutions and in complex biological samples. The sandwich assay utilizing a PIM-2-selective detection antibody featured a low limit of quantification (44 pg of active recombinant PIM-2). Fluorescent probes were efficiently taken up by U2OS cells and showed a high extent of co-localization with PIM-1 fused with a fluorescent protein. Overall, the developed inhibitors and derivatives represent versatile chemical tools for studying PIM function in cellular systems in normal and disease physiology. 相似文献
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