Untersuchung von Einzelblasen an ölbeschichteten Oberflächen mit künstlichen Keimstellen

Zusammenfassung An feinpolierten, ölbeschichteten V2A-Oberflächen mit jeweils einer gebohrten (d=140 µm) und einer geätzten (d=47 µm) Vertiefung als Keimstelle wurde für Wasserblasen das Wachstum, der Abreißdurchmesser und die Abreißfrequenz gemessen. Für die wärmetransportgesteuerte Blasenbildung liefert der Vergleich mit Beziehungen, die für reine Metallflächen gewonnen wurden, gute Übereinstimmung, so daß keine Einfluß der Ölschicht erkennbar wurde.

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Investigation of single vapor bubbles on surfaces covered with a thin oil film with artificial nuclei

For single vapor bubbles, the growth rates, the detachment diameter, and the frequency were measured on single artificial nuclei, either drilled (d=140 µm) or etched (d=47 µm) into a highly polished stainless steel plate which was covered with a thin oil film. For evaporation controlled by heat transport, good agreement was observed with relations from literature which were obtained for clean surfaces; thus, an effect of the oil film could not be discovered.

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Formelzeichen a Temperaturleitfähigkeit - D _a Blasenabreißdurchmesser - f Blasenfrequenz - Ja Jakob-Zahl - Pr Prandtl-Zahl /a - q Wärmestromdichte - R Blasenradius - T Temperatur - dynamische Zähigkeit - Wärmeleitfähigkeit - Dichte - Oberflächenspannung - Zeit Indizes g Gas, Dampf - l flüssig - s Sättigung - w Wand Herrn Prof. Dr.-Ing. Karl Stephan zum 60. Geburtstag gewidmet     

High-Sensitivity Immunofluorescence Staining: A Comparison of the Liposome Procedure and the FASER Technique on mGR Detection

Flow cytometry has become a widely-used and powerful tool for the characterization of cells according to their expression of specific proteins. However, sensitivity of this method is still limited since conventionally labeled antibodies can be conjugated with at maximum 1–10 dye molecules. This fact resulted in the need to develop new techniques in order to identify molecules which are expressed in very low but functionally relevant amounts. In the past, we have successfully used a liposome-based high-sensitivity immunofluorescence technique to measure the expression of low abundant membrane bound glucocorticoid receptors (mGR) on different cell types. The use of this technique allows the detection of as few as 50–100 antigen molecules per cell which is due to a 100-fold to 1000-fold increase in fluorescence signal intensity compared with conventional methods. The higher sensitivity is achieved since thousands of dye molecules can be enclosed in liposomes. Another modern high-sensitivity immunofluorescence staining method is the purchasable Fluorescence Amplification by Sequential Employment of Reagents (FASER) procedure. Here, we aimed at comparing sensitivity and specificity of these two techniques for the detection of the mGR. Our data demonstrate the FASER technique to be more sensitive and also more specific for the detection of mGR as compared to the liposome technique. However, both methods have advantages and disadvantages which are discussed in detail.     

Lattice electrons on a cylinder surface in the presence of rational magnetic flux and disorder

We consider a disordered two-dimensional system of independent lattice electrons in a perpendicular magnetic field with rigid confinement in one direction and generalized periodic boundary conditions (GPBC) in the other direction. The objects investigated numerically are the orbits in the plane spanned by the energy eigenvalues and the corresponding center of mass coordinate in the confined direction, parameterized by the phase characterizing the GPBC. The Kubo Hall conductivity is expressed in terms of the winding numbers of these orbits. For vanishing disorder the spectrum of the system consists of Harper bands with energy levels corresponding to the edge states within the band gaps. Disorder leads to broadening of the bands. For sufficiently large systems localized states occur in the band tails. We find that within the mobility gaps of bulk states the Diophantine equation determines the value of the Hall conductivity as known for systems with torus geometry (PBCs in both directions). Within the spectral bands of extended states the Hall conductivity fluctuates strongly. For sufficiently large systems the generic behavior of localization-delocalization transitions characteristic for the quantum Hall effect are recovered.     

Continuous bordering of matrices and continuous matrix decompositions

Summary. Let be the set of all real -matrices of rank . We prove that for there are no continuous vector fields such that the bordered matrix is regular for all . This result has some relevance for the numerical analysis of steady state bifurcation. As a by-product we show that there is no nonvanishing continuous vector field with for all , where is the set of all matrices of rank deficiency one. This implies that there is no singular value decomposition of depending continuously on in any matrix set which contains . As another application we prove that in general there is no global analytic singular value decomposition for analytic matrix valued functions of more than one real variable. Received October 6, 1993 / Revised version received July 18, 1994     

Physicochemical investigation of a lipid with a new core structure for gene transfection: 2-amino-3-hexadecyloxy-2-(hexadecyloxymethyl)propan-1-ol

Cationic liposomes/DNA complexes can be used as nonviral vectors for direct delivery of DNA-based biopharmaceuticals to damaged cells and tissues. In order to obtain more effective and safer liposome-based gene transfection systems, the new cationic lipid 2-amino-3-hexadecyloxy-2-(hexadecyloxymethyl)propan-1-ol (AHHP) was synthesized. In this paper we report on the synthesis of AHHP and investigations of its physical-chemical properties. Langmuir monolayers of AHHP were studied at the air/buffer interface by film balance measurements, grazing incidence X-ray diffraction (GIXD), and infrared reflection absorption spectroscopy (IRRAS). Structure and thermotropic phase behavior of AHHP in aqueous dispersion were examined by small-angle and wide-angle X-ray scattering (SAXS/WAXS) and differential scanning calorimetry (DSC). The results show clear differences in structure and phase behavior of AHHP, both in the monolayer system and in aqueous dispersions, in dependence on the subphase pH due to protonation or deprotonation of the primary amine in the lipid head group. Thermodynamic data derived from pi-A isotherms provide information about the critical temperature (Tc), which is in rough agreement with the temperature of the lipid phase transition from gel to fluid state (Tm) found by X-ray and calorimetry studies of AHHP aqueous dispersions. The packing properties of the molecules in mono- and bilayer systems are very similar. DNA couples to the monolayer of the new lipid at low as well as at high pH but in different amounts. The DNA coupling leads to an alignment of adsorbed DNA strands indicated by the appearance of a Bragg peak. The distance between aligned DNA strands does not change much with increasing monolayer pressure.     

Functionalization of bolalipid nanofibers by silicification and subsequent one-dimensional fixation of gold nanoparticles

In the present work, we describe the successful stabilization of bolalipid nanofibers by sol-gel condensation (silicification) of tetraethoxysilane (TEOS) or 3-mercaptopropyltriethoxysilane (MP-TEOS), respectively, onto the nanofibers. The conditions for an effective and reproducible silicification reaction were determined, and the silicification process was pursued by transmission electron microscopy (TEM). The resulting bolalipid-silica composite nanofibers were characterized by means of differential scanning calorimetry (DSC), TEM, (13)C, and (31)P NMR spectroscopy. Finally, the novel silicified bolalipid nanofibers were used as templates for the fixation of 5 and 2 nm AuNPs, respectively, resulting in one of the rare examples of one-dimensional AuNP arrangements in aqueous suspension.     

Systematic studies on the determination of Hg-labelled proteins using laser ablation-ICPMS and isotope dilution analysis

A method was developed for the precise and accurate determination of ovalbumin labelled with p-hydroxy-mercuribenzoic acid (pHMB) using polyacrylamide gel electrophoresis with ns-laser ablation–inductively coupled plasma mass spectrometry. Following systematic optimisation of the ablation process in terms of detection sensitivity, two different quantification strategies were applied: external calibration using standards of the derivatized protein after ¹³C⁺ normalization and, as a proof of concept, label-specific isotope dilution analysis (IDA) using pHMB enriched in the isotope ¹⁹⁹Hg. Due to the inhomogeneous distribution of the protein within the gel bands, it could be demonstrated that the IDA approach was superior in terms of precision and accuracy. Furthermore, it permits a reliable quantification, if more complex separation protocols are applied, as typically occurring analyte loss and degradation can be compensated for as soon as complete mixture of spike and sample is achieved. The estimated limit of detection was 160 fmol in the case of ovalbumin. In contrast to earlier studies using metals naturally present in proteins, no loss of mercury was observed during separation under denaturing conditions and other sample preparation steps. Using label-specific IDA, the measured isotope ratios in the gel corresponded to recoveries between 95% and 103%.