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Peter D. Senter Marilyn J. Tansey John M. Lambert Walter A. Blattler 《Photochemistry and photobiology》1985,42(3):231-237
Abstract— Two photolabile heterobifunctional protein crosslinking reagents have been synthesized and used for the conjugation of a protein toxin to an antibody. o-Nitrobenzyl alcohol derivatives containing protected sulfhydryl groups were converted to o-nitrobenzyloxycarbonyl chlorides and covalently attached to pokeweed antiviral protein (PAP-S) obtained from the seeds of Phytolacca americana. The sulfhydryl groups were deprotected and the modified toxins were reacted with J5 antibody (specific for the common acute lymphoblastic leukemia antigen, CALLA) that had been functionalized with maleimido groups. Antibody-toxin conjugates were formed predominantly in the ratio of 1:1, and were purified from unconjugated antibody and PAP-S. Irradiation of the conjugates with light having a peak intensity at 365 nm effected photolytic fragmentation, and PAP-S was released in fully active form. The methods described here may prove useful for the release of drugs or toxins at sites accessible to light. 相似文献
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