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Karthik Arumugam Mallikarjuna Rao Chamallamudi Ravindranath Reddy Gilibili Ramesh Mullangi Subramanian Ganesan Sidhartha S Kar Ranjithkumar Averineni Gopal Shavi Nayanabhirama Udupa 《Biomedical chromatography : BMC》2011,25(3):353-361
A sensitive, specific and accurate HPLC method for the quantification of rivastigmine (RSM) in rat urine was developed and validated. The method involves the simple liquid–liquid extraction of RSM and pyridostigmine as an internal standard (IS) from rat urine with tertiary methyl butyl ether. The chromatographic separation of RSM and IS was achieved with 20 mm ammonium acetate buffer (pH 6.5) and acetonitrile (65:35, v/v) delivered at flow‐rate of 1 mL/min on a Kromasil KR‐100. The method was in linear range from 50 to 5000 ng/mL. The validation was done as per FDA guidelines and the results met the acceptance criteria. The method was successfully applied for the quantification of RSM in rat urine. Besides method validation, we have identified two metabolites of RSM in urine. Both the metabolites were characterized by HPLC‐PDA and LC‐MS/MS and it was found that one metabolite is novel. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
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Maheshwari Gourab Subramanian Ganesa Sundararajan Karthik Arumugam Ranjithkumar Averineni Ginjupalli Prashant Musmade Kishore Udupa Nayanabhirama 《平面色谱法杂志一现代薄层色谱法》2007,20(5):335-339
JPC – Journal of Planar Chromatography – Modern TLC - A sensitive, accurate, precise, and stability-indicating high-performance thin-layer chromatographic method has been established... 相似文献
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