排序方式: 共有4条查询结果,搜索用时 0 毫秒
1
1.
Carvalho Andrade Carolina M. M. Aguiar Wilson Bucker Antranikian Garo 《Applied biochemistry and biotechnology》2001,91(1-9):655-669
Xylanases (EC3.2.1.8) catalyze the hydrolysis of xylan, the major constituent of hemicellulose. The use of these enzymes could
greatly improve the overall economics of processing lignocellulosic materials for the generation of liquid fuels and chemicals.
The hyperthermophilic archaeon Pyrodictium abyssi, which was originally isolated from marine hot abyssal sites, grows optimally at 97°C and is a prospective source of highly
thermostable xylanase. Its endoxylanase was shown to be highly thermostable (over 100 m in at 105°C) and active even at 110°C.
The growth of the deep-sea archaeon P. abyssi was investigated using different culture techniques. Among the carbohydrates used, beech wood xylan, birch wood glucuronoxylan
and the arabinoxylan from oats pelt appeared to be good inducers for endoxylanase and β-xylosidase production. The highest
production of arabinofuranosidase, however, was detected in the cell extracts after growth on xylose and pyruvate, indicating
that the intermediate of the tricarboxylic acid cycle acted as a nonrepressing carbon source for the production of thi enzyme.
Electron microscopic studies did not show a significant difference in the cell surface (e.g., xylanosomes) when P. abyssi cells were grown on different carbohydrates. The main kinetic parameters of the organism have been determined. The cell yield
was shown to be very low owing to incomplete substrate utilization, but a very high maximal specific growth rate was determined
(μmax=0.0195) at 90°C and pH 6.0. We also give information on the problems that arise during the fermentation of this hyperthermophilic
archaeon at elevated temperatures. 相似文献
2.
Garabed Antranikian 《Nachrichten aus der Chemie》2006,54(12):1202-1206
3.
H.-G. Hundeck A. Sauerbrei U. Hübner T. Scheper K. Schügerl R. Koch G. Antranikian 《Analytica chimica acta》1990
A newly developed four-channel enzyme thermistor system is presented and its application to biotechnological process monitoring is discussed. Different sugars were detected on-line during the cultivation of Cephalosporium acremonium and Bacillus licheniformis on technical media and during a starch hydrolysis process with immobilized thermostable enzymes. Immobilized enzymes and entrapped microorganisms were used as biological compounds in this biosensor. 相似文献
4.
Applied Biochemistry and Biotechnology - Conditions were found in batch culture under which high levels of extremely thermoactive maltogenic amylase and pullulanase fromThermoanaerobacter finnii... 相似文献
1