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Hemmat Esfe Mohammad Kiannejad Amiri Mahmoud Alirezaie Ali 《Journal of Thermal Analysis and Calorimetry》2018,134(2):1113-1122
Journal of Thermal Analysis and Calorimetry - Hybrid nanofluid can be considered as a new generation of nanofluids. Despite the success of the researchers in the field of hybrid nanofluids, no... 相似文献
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The miniaturization of laboratory processes offers substantial advantages over traditional techniques in terms of cost, speed, and potential for multistage automation. To date, only a few studies have reported successful microfluidics-based immunoassays, most of which rely on fluorescence detection technologies. The goal of this study was to develop a poly(dimethylsiloxane) microfluidics-based immunoassay methodology and a versatile colorimetric quantification scheme for the detection of visual colour changes resulting from immune reactions in microchannels. The novel immunoassay technique was applied towards the detection of Helicobacter pylori infection using 20 human serum samples of known infection status, and results compared with conventional nitrocellulose membrane-based dot-ELISA. The microchannel immunoassay reliably detected H. pylori antigens in quantities on the order of 10 ng, which provides a sensitivity of detection comparable to conventional dot-blot assays. Sensitivity was 100%, specificity was 90%, positive predictive value 91%, and negative predictive value 100%, with an overall accuracy of 95%. The software developed generated results that were consistent with visual observations and by automatically taking into account background intensity changes, the software minimized subjectivity. Volumes of solutions used were 100-fold less compared with conventional immunoassays. Miniaturization of the ELISA using this technique provides a means for the accurate diagnosis of microbial infection while minimizing waste production. 相似文献
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Amir H. Haji Alirezaie Amir H. Navarchian Hassan Sabzyan 《Polymer Science Series A》2016,58(3):487-498
Barrier properties of pristine polyethylene (PE) and polyethylene/montmorillonite-clay (PE/MMT) nanocomposite films with different MMT layers configurations were studied using molecular dynamics simulation within NVT ensemble. The force field parameters were optimized for bond lengths, bond angles and torsion angles of the MMT structure. A special simulation box was designed to simulate the diffusion of oxygen, nitrogen and methane, through pristine PE and PE/MMT nanocomposite films. The diffusion coefficients of these gases and the tortuosity values were calculated and analyzed. Results showed that the configuration of clay nanoparticles has strong effect on the barrier properties of the nanocomposite films. The parallel configuration for layered silicates was predicted to have a low diffusion coefficient and a high tortuosity parameter for gas diffusive molecules. The simulation could also indicate that the diffusion coefficient of oxygen is higher than those of nitrogen and methane gases in the examined systems which can be attributed to the smaller kinetic diameter of oxygen. 相似文献
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