A Ca2+-phospholipid-dependent protein kinase from cottonplant shoots

The Ca²⁺-phospholipid-dependent protein kinase from cottonplant shoots was purified by chromatography on DEAE-Sepharose CL-6B, and then on phenyl-Sepharose CL-4B. According to electrophoresis in PAAG, the enzyme was practically homogeneous and had a molecular mass of 57 kDa. In the presence of Ca²⁺ alone, the enzyme was activated to only a slight degree. Under the combined action of Ca²⁺ and a phospholipid the action of the enzyme rose severalfold. A determination of amino acid specificity showed that the protein kinase isolated was a serine- and threonine-specific protein kinase.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax 627071. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 100–105, January–February, 1994.     

Activation by a cytokinin-binding protein of the phosphorylation reaction; Synthesis of RNA and protein in cottonplant chromatin

The dependence of protein kinase and RNA polymerase activities, and also of the synthesis of protein in nuclear chromatin, on the addition of benzylaminopurine and a cytokinin-binding protein to the incubation mixture has been investigated. It has been shown that all three processes are hormone-dependent. It has been found that the activity of protein kinase C is regulated by a cytokinin.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 62 70 71. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 745–749, September–October, 1997.     

Interaction of 1-acylamino-2,2-dichloroethenyl(triphenyl)phosphonium chlorides with alkanolamines

Abstract

It is shown that the interaction of 1-acylamino-2,2-dichloroethenyl(triphenyl)-phosphonium chlorides with alkanolamines having a primary amino group results in the formation of 4-oxazolylphosphonium salts containing hydroxyalkylamine substituents at position 5 of the oxazole cycle. Under similar conditions the reaction of N-substituted alkanolamines with 1-acylamino-2,2-dichloroethenyl-(triphenyl)phosphonium chlorides leads to the formation of 1,3-oxazolidin-2-ylidene derivatives, in which the triphenylphosphonium group is located in the side chain. The structure of the new synthesized compounds has been reliably proven by elemental analysis, IR, ¹Н, ¹³С, ³¹Р NMR spectroscopy, mass spectrometry and single crystal X-ray diffraction.     

Clathrate [Zn(C<Subscript>6</Subscript>H<Subscript>5</Subscript>COO)<Subscript>2</Subscript>(H<Subscript>2</Subscript>O)<Subscript>2</Subscript>] · 2CH<Subscript>3</Subscript>COOH: Synthesis and crystal structure

The clathrate [Zn(C₆H₅COO)₂(H₂O)₂] · 2CH₃COOH (I) was obtained for the first time from zinc(II) benzoate. The individuality, the unit cell parameters, and the number of “guest” molecules in complex I were determined from X-ray diffraction and derivatographic data. Its crystal structure was solved.     

Radical Polymerization of N-Vinylcaprolactam in Isopropanol

Radical polymerization of N-vinylcaprolactam in isopropanol, initiated with azobis(isobutyronitrile), was studied with the aim to prepare polyvinylcaprolactam of controllable molecular weight. The rate and degree of polymerization are influenced by the reaction mixture composition.     

1,3-oxazole derived cytisines

Reactions of 1-acylamino-2,2-dichloroacrylonitriles, diethyl 1-acylamino-2,2,2-trichloroethyl-phosphonates and 1-acylamino-2,2-dichloroethenylphosphonium salts with natural alkaloid cytisine have been studied. A series of novel multifunctional cytisine derivatives containing pharmacophore 1,3-oxazole moiety have been obtained. Composition and structure of the synthesized compounds have been confirmed by mass spectrometry and NMR spectroscopy data.     

Phosphorylation of proteins of isolated cotton plant chloroplasts under the action of various phytohormones and various concentrations of magnesium ions

cAMP and Ca phospholipid-dependent protein kinases have been detected in isolated cottonplant chloroplasts. A change in the activity of these enzymes in the presence of phytohormones and of various concentrations of magnesium ions has been shown.Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 138–141, January–February, 1995. Original article submitted October 17, 1994.