烟瘤B6S3细胞和矮牵牛W43属间体细胞杂种植株再生及LpDH的表达

本文利用烟草冠瘿瘤生长激素自主生长、不能再建形态及含有章鱼碱合成酶的特点,和具有截然相反特性的矮牵牛叶片原生质体建立了体细胞杂种筛选体系。由两者融合的原生质体中,选出2％的能持续生长的绿色愈伤组织。由它们分化出的一些幼苗形态倾向于矮牵牛,但有21株含有烟瘤特有的合成章鱼碱的合成酶,表明为体细胞杂种性质。     

SOMATIC HYBRID PLANTS FROM INTERGENERIC FUSION BETWEEN TOBACCO T

Tobacco tumor B6S3 characterized by phytohormones-independence and incapacity to re-generate plant and containing genetic marker-LpDH has been fused with Petunia hybridaW43 possessing the properties in contrast with the tobacco tumor. Using these characteris-tics, we have established a selective system of somatic hybrid in protoplast fusion, andscreened 2% of the continuously growing green calli from fusion products. Twenty-onehybrids from thirty-four regenerated plants are morphologically inclined to Petunia, but thepresence of lysopine dehydrogenase activity in these plants shows that they are of hybridnature.