The Production of Monoclonal Antibody to Androstenedione and the Effect of Passive Immunization on Xinjiang Fine-Wool Sheep With It

Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedione-11α-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1—12.3%) and estrone (0.8—2.5%) and high affinities ranging from 2.0×10~7 to 2.8×10~8 L/M. They were all of the IgG_1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.     

雄烯二酮单克隆抗体的制备及其被动免疫新疆细毛羊的效应

本文应用人工合成的雄烯二酮11α-半琥珀酰-牛血清白蛋白为抗原,建立了5株稳定分泌抗雄烯二酮特异性单克隆抗体(McAb)的杂交瘤细胞株。McAb与睾酮、雌酮交叉反应分别为8.1—12.3％和0.8—2.5％,抗体亲和力为2.0 x10~7—2.8×10~8L/M之间。它们均为IgG_1亚类。使用该McAb被动免疫新疆细毛羊,两个情期中孕酮(P)和促黄体激素(LH)的含量皆高于对照组,绵羊排双卵和产双羔都有所提高。