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人参花粉植株的诱导及体细胞无性系的建立   总被引:6,自引:0,他引:6  
人参花药培养已连续四年(1981—1984年)得到了花粉植株,并建立了体细胞无性系。人参花药对培养基的适应较广,在MS等6种基本培养基上都得到了愈伤组织;低温预处理能明显提高诱导效果;已筛选出愈伤组织诱导率高的培养基配方,再生植株的分化比较困难,在100多种分化培养基配方中筛选出16种能分化再生植株的配方,分化率高的可达6.8%。经二年多16次继代培养,建立了能保持良好分化能力的体细胞无性系。  相似文献   
2.
In anther culture of Panax ginseng, its callus formation showed a wide adaptability to culture media. Large numbers of calli were induced on media exhibiting better effects of induction. Supplemeres of 5 mg 2,4-D/1 and 1 mg KT/1 to the media proved to be much effective. Regeneration of the whole plantlets from anther culture of Panax ginseng is usually quite difficult. During the past three years, however, sixteen of the 100 medium formulae tested were proved to be suitable. The formulae of MS+0.5 mg BA/1+2 mg GA/1+1000 mg LH/1+3% sucrose were considered good and effective. A visibly differentiated body, which was light-milky white and later turned into a light green spot, was formed 40 days after the callus was transferred to the differentiation media. This body differentiated subsequently into buds, roots and, eventually, seedlings. The embryoid clones have been established in order to maintain its ability of continual differentiation into plantlets through successive culturings of many generations.  相似文献   
3.
吴刚  陈泽光 《实验力学》1994,9(2):165-169
本文对具有不同裂纹尺寸的中心裂纹LY12板试样在动、静态拉伸加载条件下,分别进行了断裂实验研究,得到了名义断裂应力与板的初始裂纹长度间的关系。通过对动、静态实验结果的比较,得到在动、静态拉伸加载条件下某些参量间的相互关系。  相似文献   
4.
作者对45钢等三种延性金属材料进行了人字形切槽短小柱体平面应变断裂韧性K_(IC)测试法的实验研究.对于这三种延性材料,测试实验结果出现了显著的尺寸效应.分析表明:这种K_(IC)测试法不宜用于这些延性金属材料.  相似文献   
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