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从人、Homo sapiens、胚胎组织及细胞核的抽提物中分离纯化了一种核酸内切酶HsaI。它具有Ⅱ类核酸内切酶的功能、从该酶对λDNA的切割位点来看,它是EcoRI的同功酶,但在洗脱特性上不同于EcoRI。经SDS-聚丙烯酰胺凝胶电泳分析可得两条考马氏兰染色带,分子量分别为65000和22000 Daltons。 相似文献
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Gene libraries of Rana nigromaculata and Mus musculus (C_(57)J_6) have been constructed bythe cosmid system developed by Collins using BamHl and Pstl as cloning restriction enzymes.A simplified formula, which is a modification of Clarke and Carbon derived from apprixima-tion, was used for calculating the number of colonies or recombinants necessary for theconstruction of a gene library. Pro~ and leu~ complementary to the recipient cells (RB101)with the genetic constitution of pro~- and leu~- have been selected from the BamHl genelibraries of both Rana nigromaculata and the mouse in the present work. 相似文献
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The HsaI restriction enzyme from the embryos of human, Homo sapiens, has been isolated with both the tissue extract and nnelear extract. It proves to be an unusual enzyme, clearly related functionally to Type Ⅱ endonuclease. HsaI seems to be an isoschizomer of EcoRI, but it has a distinctive property of elation, differing from EeoRI. Upon SDS-polyacrylamide gel electrophoresis, the enzyme preparation showed Coomassie blue staining bands, having the molecular weight of 65,000 and 22,000 daltons in size, respectively. 相似文献
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