cAMP及其衍生物金属离子铬合物的核磁共振研究

本文报导用核磁共振谱研究了有重要生理意义的二价金属离子与cAMP及其衍生物双丁酰cAMP的相互作用,比较了环苷酸与金属络合时各谱线的化学位移值和谱线宽度。     

Applications of Oblique-Incidence Reflectivity Difference Method in Primary Study of Protein Biomolecules

Oblique-incidence reflectivity difference （OI-RD） analysis is applied to detect the immunoglobulin-G and cytochrome biomolecules on standard glass substrates without fluorescence labelling. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential applications in detection of biochemical processes.     

激光作用下血红蛋白的荧光

一、引言应用激光光解研究生物大分子的光解动力学过程,已做了不少工作.但对激光作用下蛋白质分子的非线性光学现象,还研究的不多.我们应用自己设计安装的激光实验装置,以血红蛋白和碳氧血红蛋白为对象,研究它们在激光作用下的光解离和双光子过程、荧光随时间变化等现象.我们观察到,在激光作用下,碳氧血红蛋白和血红蛋白出现双光子过程和发短波长荧光,而在碳氧血红蛋白中出现“滞后”荧光现象.这些现象可对生物分子内部能级和能量转移过程等提供有益的信息.     

Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents

This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference （OI-RD） analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.