DIRECT SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM PROTOPLAST-DERIVED CELLS OF WHEAT (Triticum aestivum L.)

Protoplasts were isolated from embryogenic calli derived from immature embryos ofwheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB mediumsupplemented with 1mg/L 2,4- D and 500mg/l casein hydrolysate (CH). The regenerated cellsfrom protoplasts divided to form somatic embryos directly. The somatic embryos grown to1.5- 2 mm in size directly developed into complete plants on solid MB medium without hor-mones.     

小麦原生质体再生细胞直接形成体细胞胚和再生植株

本文用普通小麦品种“济南177”幼胚诱导产生的胚性愈伤组织为材料游离原生质体,培养在附加有1mg/12,4-D和500mg/1水解酪蛋白(CH)的NMB培养基中,原生质体再生细胞直接分裂成为体细胞胚。生长至1.5—2mm大小的体细胞胚在无激素的MB固体培养基上直接发育成为完整再生小植株。