Relationship Between Electron Transport or Proton Transduction and ATP Synthesis in Fusion Membranes in Light

The phosphorylation coupling factors of the chloroplast thylakoid membranes were plucked off to form deficient membranes which were combined with crista membranes to form a fusion system. This membranes reconstituted ATP synthesis activity in light. However, the △pH in the reaction medium was not observed.This paper is a report on some of the results obtained recently in connection with the problem. Various examinations and measurements were used and this phenomenon was repeated. Through the reconstituted system, (thylakoid membranes combined with crista membranes) the kinetic proton changes were studied. It proved that the photophosphorylation activity of this system increased with the increase of the quantity of the combined crista membranes to which, however, the △pH in medium, the effects of 9-AA fluorescence quenching, and the absorption change of neutral red in the membrane pockets were inversely proportional. For this phenomenon, analyses and discussions on the relationship between electron tra     

融合膜光下电子传递、质子传导与ATP合成的关系

融合膜重建ATP合成,而未见介质△pH,本文利用不同检测手段,重现了这一现象。通过类囊体膜与嵴膜的组合膜系,研究了光下质子动态变化,证明了这种膜系的光合磷酸化活力有随嵴膜组合量的增加而增加的现象,而介质△pH,9-AA荧光淬灭效应,和囊内中性红吸收变化却相反,随嵴膜组合量的增加而下降。对此现象,从融合膜中电子传递、质子传导与ATP合成的关系进行了分析和论证。