乙型肝炎病毒adr NC-1 DNA的全顺序测定

在建立剪切重组法与内引物法连续、快速测定大片DNA完整顺序的基础上,用定点克隆与随机克隆相结合的实验设计,以末端终止法测定了HBV adr NC-1 DNA全顺序。全基因组长3195核苷酸,390核苷酸以上的框架有5个,其余都在300以下,框架V只在NC-1中发现,在其它HBV中尚未见报道。pre s与x基因的起点NC-1也与其它亚型不同。基因重叠与碱基重复使用的规律与其它病毒的表现相同。     

DETERMINATION OF THE COMPLETE NUCLEOTIDE SEQUENCE OF HBV adr NC-1 DNA

On the basis of sequencing the large DNA-fragments which have been inserted intoM_(13)mp_8, we design a simple strategy to determine the complete nucleotide sequence of HBVadr NC-1 DNA with chain termination method. The whole genome is 3195 nucleotides long.Five reading frames are observed. The gene location and organization are shown.