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Spiroplasma has been successfully isolated from the honey bee (Apis mellifera) and cultivated in R-2 medium in China. The isolate, CH-1 is obtained through triple cloning. CH-1 shows a helical morphology and a contractile movement. It is 0.17 μm in diameter and its length varies considerably with different growth periods. CH-1 forms granular-type and/or fried-egg-shaped colonies, 75—210 μm in diameter, on R-2 medium. It grows normally at temperatures ranging 25—35℃ (the optimum being 28—32℃). The optimal pH for growth is 7.0. The best growth occurs in medium containing 0.25 mol/L of sucrose. Under the optimal condition, its growth reaches the stationary phase 48 h after subculture. CH-1 cannot grow in serum-free medium and its growth is inhibited by digitonin. CH-1 can metabolize glucose, fructose, maltose, and trehalose. It can dehydrate arginine, CH-1 does not have an ability to utilize urea, nor dehydrate gelatin, nor reduce methylence blue. The taxonomic position of CH-1 has also been discuss 相似文献
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蜜蜂螺原体基本性状的研究 总被引:8,自引:0,他引:8
在我国首次用R-2培养基从蜜蜂上分离到的一个螺原体分离物CH-1,具有典型的螺原体形态结构和运动性。菌体直径0.17μm,长度随不同的生长时期而变化。在固体R-2培养基上形成直径75—210μm的煎蛋形或圆形菌落。CH-1生长温度范围25—37℃,最适28—32℃;最适生长pH7.0;渗透压0.25mol/L蔗糖浓度;在最适生长条件下,48h达到生长高峰。CH-1生长需要提供血清,并受毛地黄皂苷的抑制;可利用葡萄糖、果糖、麦芽糖、海藻糖和精氨酸;不能利用尿素;不能水解明胶;对甲基蓝无还原能力。 相似文献
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