水稻组蛋白H3基因和1.5-二磷酸核酮糖羧化酶/加氧酶小亚基基因的克隆和结构分析

本文从水稻(Oryza sativa,IR26)细胞核中分离出的几个基因,测定了它们的DNA核苷酸序列,并从水稻IR26基因组文库中筛选和分离到一个14.8kb的DNA片段,其中含有一个H3基因和另一个类似H3的假基因。DNA序列分析的结果表明:水稻H3基因的编码顺序有405bp长,其5′和3′端的非编码区则含有几个与一般真核基因或组蛋白基因共同的调控序列。水稻H3基因的密码子有一显著特点,密码子的第三个核苷酸98％为G和C,通过Southern转移分析,表明水稻二倍体基因组中大约有50个左右H3基因,从同一个水稻基因文库中,我们还分离鉴定到了rbcS基因,它编码1,5-二磷酸核酮糖羧化酶/加氧酶(Rubisco)的小亚基,水稻rbcS基因的顺序含有一个内含子,其位置与小麦的相同,水稻和小麦rbcS基因所编码的转移肽,其最初18个氨基酸彼此是相同的。     

CLONING AND ANALYSIS OF HISTONE 3 GENE AND RUBISCO SMALL SUBUNIT GENE OF RICE

We have isolated and determined the DNA sequence of several genes from the nucleus of rice (Oryza sativa, IR26). We screened a genomic library of rice IR26 and isolated a 14.8 kb segment containing an H3 gene and an H3-like pseudogene. Sequence analysis showed that the coding sequence of the rice H3 gene is 405 bp in length, and the 5' and 3' noncoding regions contain several regulatory sequences common to eukaryotic or histone genes. The codon usage of the rice H3 gene is highly unusual in that the third codon position is 98% G and C. Southern blotting analysis suggested that the copy number of the H3 gene is around 50 per diploid rice genuine. From the same rice geaomic library, we have identified rbcS, which codes for the small subunit of ribulose-1, 5-bisphosphate carboxylase-oxygenase (Rubisco). The rbcS sequence is interrupted by an intron at the same location in both rice and wheat. The first 18 amino acids of the transit peptide in rice and wheat rbcS are identical