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为阐明稀土离子(Ln3+ )对胰岛素的跨膜转运和降糖作用的机理, 通过谱学方法研究了Ln3+与去锌和含锌胰岛素的相互作用. 结果表明Ln3+与胰岛素的结合诱导胰岛素从二级到高级结构的改变与其浓度相关. 在低浓度下, 它们诱导胰岛素单体分子内的构象变化. 变化涉及它与胰岛素受体结合区域(B(24~30)). 这可能与Ln3+在低浓度下增强胰岛素与其受体结合有关. Ln3+还能促进胰岛素单体之间的聚集. Ln3+诱导胰岛素聚集的能力大于Zn2+ . 这种聚集可以用EDTA解聚而部分恢复. 提示Ln3+诱导的聚集不是变性聚集. 与Zn2+ 相似, 在一定的浓度范围内, Ln3+具有稳定胰岛素的作用, 但强于Zn2+ . 相似文献
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To clarify the mechanism of lanthanide ions (Ln3+) on the across-membrane transport of insulin and subsequent reducing blood glucose, the interactions of Ln3+with Zn-insulin and Zn-free insulin are investigated by spectroscopic methods. The results reveal that the binding of Ln3+ to insulin can induce its structure changes from secondary to quaternary structure, depending on the Ln3+ concentration. In the lower concentration, it triggers the conformational changes of insulin monomer in the binding region with insulin receptor (B(24-30)). It would affect insulin-insulin receptor interaction. Moreover, Ln3+ binding promotes the assembly of insulin monomer from dimer to polymer. The potency of Ln3+ in inducing insulin's aggregation is stronger than that of Zn2+. Furthermore, the aggregation can be reversed partly by EDTA-treatment, indicating that it is not due to denaturation. Similar to Zn2+ effect, Ln3+ can stabilize insulin hexamer in a certain range of concentration, but is stronger than the former. 相似文献
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用质子诱导X射线发射法测定人红细胞与Gd柠檬酸和乳酸络合物温育时,胞浆,细胞膜膜脂和膜蛋白上Gd含量随时间的变化及阴离子通道抑剂对Gd进入细胞的影响。 相似文献
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Tb3+与氧合血红蛋白反应动力学的停流荧光光度法研究 总被引:3,自引:0,他引:3
近年来,稀土在农业中的应用引起人们对它的安全性的关注.:Evans口'曾报道稀土化合物由于其类Ca2+性导致各种负面生物效应,包括影响心血管功能及溶血.然而有关稀土离子与血红蛋白的研究工作国内外尚很少见报道. 相似文献
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