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Plasma membranes prepared from pig, mouse and rat liver incubated with insulin resulted in the release of at least two insulin chemical mediators. These mediators, identified as fractions 1 and 3, were found to inhibit cAMP level in response to lipolytic hormone and forskolin and to enhance lipogenesis in adipocytes of rat. Fractions 1 and 3 have been estimated to have molecular weights of 3700—4000 and 1000—1500 dalton, respectively. This initial report will focus on fraction 1.Interestingly, liver membranes from diabetic animals were found not to release mediators in the presence of insulin. However, following in vivo treatment of diabetic animals with insulin, the liver membranes appeared to restore its ability in generating chemical mediators in response to insulin. 相似文献
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本文采用八步分离提取法,成功地从2,800,000个家蚕(Bombyx mori)雄蛾头部提取和初步纯化了脑激素——即促前胸腺激素(PTTH),称为“粗制PTTH”.脑激素粗提物约7微克经蓖麻蚕无脑蛹的测试表明具有生物活性.又由100,000个家蚕蛾头分离提取的粗制PTTH,再经过四步凝胶柱层析进一步纯化,结果获得3.4毫克更纯的脑激素(“高纯度PTTH”).用7毫微克高纯度PTTH,即可使去脑蓖麻蚕施发育为成虫. 相似文献
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胰岛素介体的功能——调节细胞的cAMP和脂肪合成及糖尿病的影响 总被引:3,自引:0,他引:3
本文报道用猪、小白鼠和大白鼠肝细胞质膜与胰岛素(100—150μU/ml)保温后,从膜至少释放两种胰岛素介体(Insulin mediator或Insulin chemical mediator),即组份1和组份3。它们能抑制脂解激素和福司可林(Forskolin)所刺激的脂肪细胞的cAMP形成及促进细胞的脂肪合成。组份1的分子量约为3700—4000,组份3约1000—1500dalton。组份1胰岛素介体为本文第一次报道的新发现的化合物。糖尿病小白鼠或大白鼠的肝细胞膜产生此种介体比正常动物的明显下降,但经体内注射胰岛素治疗后,则介体的释放可恢复正常。 相似文献
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Incubating plasma membranes prepared from pig liver with varying concentrations of insulin (50—1000 μU/ml) resulted in the release of at least two insulin chemical mediators. One of them was fraction 1 of insulin mediator (M. W. 3700—4000 daltons) which had a significant lipogenesis-stimulatlug activity. The other was fraction 2 of insulin mediator (M. W. about 1000 daltons) which exhibited a lipogenesis-inhibitory activity.The ratio of yield between the two mediators produced from the membranes was not only dependent on the concentration but also on the potency of insulin and its analogs added. The result showed that there was more production of fraction 2 than fraction 1 with the inducer at low concentration (100 μU/ml), while the production of fraction 1 from the plasma membranes incubated with high concentration of insulin (300 μU/ml) was higher than fraction 2. On the other hand, insulin and its analogs which have different biological activities and receptor bindtng activities have been used t 相似文献
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