正交晶型江浙蝮蛇毒碱性磷脂酶A2初步结晶学研究

The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9.175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolution using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewer molecules in the asymmetric unit.     

微重力下的蛋白质晶体生长

空间微重力环境是研究复杂的蛋白质结晶过程和生长高质量蛋白质晶体的理想地方。于1992年8月应用国内研制的装置,在返回式卫星FSW-2上完成了空间蛋白质晶体生长实验。有6种蛋白质在空间长出了晶体,其中蛇毒酸性磷脂酶A_2和鸡蛋清溶菌酶的晶体明显优于地面对照实验长出的晶体,表明空间的微重力利于改进蛋白质晶体的大小、完整性、形态及内部有序性。