葡聚糖免疫磁性毫微粒的制备及作为复合靶向载体研究

用共沉淀法制备出具有超顺磁性的葡聚糖磁性毫微粒.研究了制备过程中葡聚糖浓度、铁盐用量、搅拌速度、氨水浓度和Fe～(3+)/Fe～(2+)摩尔比对葡聚糖磁性毫微粒有效粒径的影响.抗人乳腺癌单抗与高碘酸钠氧化的葡聚糖磁性毫微粒反应形成葡聚糖免疫磁性毫微粒,并对它的磁化率、形态和抗体保留活性等性质进行了研究.通过放射免疫实验考察葡聚糖免疫磁性毫微粒体外结合肿瘤抗原的能力,同时研究了放射性标记的葡聚糖免疫磁性毫微粒在动物体内的磁性和抗体导向能力.     

Preparation of dextran immunological magnetic nanoparticles and their application to combined targeting carrier

Superparamagnetic dextran nanoparticles were prepared by coprecipitation. Effects of concentration of dextran, amount of ironic salts, stirring speed, concentration of ammonium hydroxide and mole ratio of Fe3+/Fe2+ on the effective diameter of dextran magnetic nanopartides (DMNP) were studied. Dextran immunological magnetic nanoparticles (DIMNP) were formed by the reaction of the monoclonal anti-human mammary cancer antibody with DMNP oxidized by sodium periodate, and the properties of magnetic susceptibility, shape and retention of antibody activity were investigated. The in vitro cancer antigen binding ability of DIMNP was demonstrated by radioimmunoassay, and the in vivo magnetic localization and antibody targeting ability of radiolabeled DIMNP were studied.     

铈对人膀胱癌细胞基质金属蛋白酶-9表达及活性的影响

以体外培养的人膀胱癌细胞为研究对象,采用四唑盐比色检测法、明胶酶谱法研究Ce4+对人膀胱癌细胞的存活、基质金属蛋白酶 9表达与活性的影响。结果表明,低浓度稀土(0 01mmol·L-1)对细胞的生长没有显著影响,但强烈抑制基质金属蛋白酶 9的表达,而此浓度Ce4+能提高基质金属蛋白酶 9的活性。高浓度稀土(1 0mmol·L-1)对细胞的生长、基质金属蛋白酶 9表达与活性均有显著的抑制作用。     

扭smash积的整体维数

建立了H-双模代数A和它的扭smash积A＊H的整体维数之间的关系.