THE ASSAY FOR THYROID STIMULATING IMMUNOGLOBULIN USING CULTURED HUMAN THYROID CELLS AND ITS CLINICAL APPLICATION

A new technique for the determination of the serum level of thyroid stimulating immunoglobulins(TSI) is described. TSI may be detected by measuring cyclic AMP increases in cultures of isolatedthyroid epithelial cells in response to added normal or patient's serum. Our results showed that the serum level in the normal control group was mostly lower than 110%,however, in 9.1% of normal human sera, TSI showed a positive result. In abnormal position, activityof TSI was higher than 110% of the rate of the normal control group. The positive result was 86%and the serum TSI levels were 270±176.6% in patients with Graves' disease before receiving antithy-roid drug. After treatment with antithyroid drugs from one to thirty months, the positive results dropp-ed to 35% and the serum TSI level decreased to 117.0±113%, which was significantly lower thanthat in untreated patients. In conclusion, TSI can be detected in the majority of the sera in patients with Graves' disease usingthis technique. It is kelpful to the diagnosis and treatment of the Graves' disease.     

用人甲状腺细胞测定TSI的实验及其应用

本文报道了单层培养人甲状腺上皮细胞的方法及应用这种细胞来测定正常人和Graves氏病患者血清中的甲状腺刺激免疫球蛋白(TSI)活性。实验结果表明正常人血清TSI水平多数小于110％,仍有9.1％的阳性率。在Graves氏患者中没有经过治疗者TSI％为270±176.6,阳性率达86％。而经抗甲状腺亢进药物治疔2.5年以上,患者血清TSI水平降至正常。测定血清TSI水平对Graves氏病患者诊断及治疗有一定意义。