In vitro PRODUCTION OF HAPLOID PLANTS FROM MULBERRY (Morus) ANTHER CULTURE

Anthers with pollen during the uninucleate to late uninucteate stages are inoculated into dedifferentiation medium, cultured at 28℃ in the dark for 15 days, and then placed under the illumination of 2000 lux 12 h a day. After 35 days, embryoids arc induced, the induction frequency reaching 13.6％. On greening, the embryoids about 3 mm in diameter are transferred to differentiation medium, and the regeneration percentage of plantlets is 37.21％. Plantlets about 2—3 cm in height are transferred first to a rooting medium for 2—4 days, then to a hormoneless medium. The formation frequency of roots is up to 95％. Chromosome examination shows that all embryoids are haploid, and the haploid plant is very successfully induced from mulberry anther culture.     

桑树花药培养获得单倍体植株

本文取花粉发育为单核至单核靠边期的花药,接种于脱分化培养基,28℃黑暗培养15日,后每日用2000 Lux光照12h,35日后形成胚状体,其诱导率可达13.6％;当胚状体长至3mm,颜色开始转绿后转移至分化培养基,分化绿苗率可达37.21％;在苗高2—3cm时于基部切下,置发根培养基处理2—4日后转移至无激素培养基培养,发根率可高达95％以上;胚状体的染色体数均为单倍体(n=14),经植株幼叶染色体鉴定,成功地获得了桑树单倍体值株。