蛋氨酸铜对西门塔尔牛血液指标的影响

选用16头平均体质量420 kg、年龄2.5岁中国西门塔尔牛阉牛,采用随机区组设计分为4组,以混合精料和风干玉米秸秆为基础日粮,以蛋氨酸铜为铜源,在基础日粮中分别添加铜0、8、16和24 mg/kg,研究了蛋氨酸铜对西门塔尔牛血液指标的影响。结果表明,血清TG、GLU、TC、ALB和TP含量显著增加(P<0.05)。24 mg/kg组SUN明显低于对照组(P<0.05)。血清GOT、GPT、AKP、ACP、LDH差异均不显著(P>0.05)。血清Cp逐渐增加(P<0.05);SOD和GSH-px活性呈上升趋势,显著提高(P<0.05);MDA呈下降趋势(P<0.05)。16 mg/kg和24mg/kg组血清铜含量显著高于对照和8 mg/kg组(P<0.05)。根据试验结果,蛋氨酸铜添加量以8~16 mg/kg为宜。     

烟草叶肉原生质体的漂浮电介质电泳研究

本文根据电介质电泳理论,首次对单个烟草叶肉细胞原生质体进行了电介质电泳漂浮实验。对单个原生质体在频率10Hz—15MHz的范围内测定了不同电导率(12.5μs/cm—520μs/cm)下的电介质电泳频谱,并根据实验数据计算了单个原生质体的膜电容C_m,数值为0.40±0.03μF/cm~2。本文还根据实验数据对细胞电融合过程中的原生质体偶极相互作用进行了分析,得出当施加外电场E_c=1.5kV/cm的情况下,两细胞之间的偶极相互作用压力π_d在频率高于20kHz时将显著地大于膜电压产生的压力π_m,成为膜穿孔的主要原因,这将为改进细胞电融合方案提供了新的依据。     

Studies of Dielectrophoretic Spectra of Levitated Tobacco Leaf Protoplast

In this paper, we have utilized the principle of dielectrophoresis to determine the dielectrophoretic spectra of single levitated tobacco leaf protoplast at various suspending media conductivities (12.5 μS/cm-520 μS/cm) over wide frequencies (10 Hz—15 MHz). From the experimental data, the membrane capacitance of the single protoplast which was 0.40±0.03μF/cm~2 has been obtained and the dipole interaction of two protoplasts in electrofusion has also been analysed. When the external electric field E_c=1.5kV/cm was applied and the frequency was higher than 20 kHz, the dipole interaction pressure ~πd would be much larger than the membrane potential pressure ~πm, and the pressure ~πd could be the main reason for membraue pores. This conclusion will provide a new basis to improve the protocol of cell electrofusion.