THE PRIMARY STRUCTURE OF THE LACTATE DEHYDROGENASE ISOZYME M. FROM GIANT PANDA

The amino acid sequences of tryptic, chymotryptic and cyanogen bromide cleavage peptides of the lac-tate dehydrogenase isozyme M4 from giant panda have been determined. Based on the overlapping peptidesand by a comparison with the known sequence of porcine lactate dehydrogenase isozyme M subunit, thecomplete primary structure of the giant panda lactate dehydrogenase isozyme M subunit has been estab-lished. The polypeptide chain of giant panda lactate dehydrogenase isozyme M subunit consists of 331amino acid residues. There is a variance of 17 amino acid residues between the porcine and the giantpanda lactate dehydrogenase isozyme M subunits. Most of the variable residues are substitutions bychemically similar amino acids and take place at residues not related to the active center of the enzyme.     

大熊猫乳酸脱氢酶同工酶M_4的一级结构

本文对大熊猫乳酸脱氢酶同工酶M_4的胰蛋白酶水解肽以及大部分糜蛋白酶水解肽和部分溴化氰切割的肽段进行了氨基酸残基的序列测定。根据重叠肽的位置并参考猪乳酸脱氢酶同工酶M亚基的已知序列,初步确定了大熊猫乳酸脱氢酶同工酶M亚基的一级结构,它由331个氨基酸残基组成,两者相比,出现十七个残基的差异,这些差异多数是同类氨基酸间的取代,并且绝大部分发生在与酶的活性中心无关的位置上。