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A combination of microdialysis sampling with ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to quantitatively analyze the effects of organophosphorus pesticides trichlorfon on acetylcholine (ACh) and choline (Ch) in the brain of crucian carp. The mechanism of organophosphorus pesticides poisoning was preliminarily discussed,showing that the ACh content in the brain of fish increased significantly with the increase of the exposure time to trichlorfon,while the Ch content decreased. It indicated that organophosphorus pesticides inhibited the activity of cholinesterase in organisms. © 2023, Youke Publishing Co.,Ltd. All rights reserved. 相似文献
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