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高效液相色谱法同时测定强化牛奶中的维生素A和D_2 总被引:4,自引:2,他引:2
A reversed-phase high performance liquid chromatographic method for the simultaneous determination ofvitamin A and D_2 in fortified milk has been developed. A couple of serially connected columns with μ-Bonda-pak C_(18)(300 × 3. 9mm, 10μm)and Novapak C_(18)(150 × 3. 9mm, 4μm)and methanol/water(96/4)mobile phasewere used. Vitamin A and D_2 were detected at wavelengths of 325nm and 265nm, respectively. By usingsaponification procedure, the recoveries of vitamin A and D_2 were in the range of 90~110%. 相似文献
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