抗4-氧赖氨酸的烟草愈伤组织突变体及其再生植株组织的研究

本文用烟草叶片发生的愈伤组织,相继用L-4-氧赖氨酸(OL,1克/升),2％甲基磺酸乙酯(EMS)和OL(4克/升)处理,选择并建立了抗OL的烟草愈伤组织突变体(W_0),它对乙硫氨酸(Eth)和5-甲基色氨酸(5MT)不表现交叉抗性。它比其亲本积累赖氨酸多一倍。从它再生出突变植株,在培养瓶内开花结籽。从突变植株发生次级愈伤组织(BCB)。检查了W_0和BCB对OL的抗性,赖氨酸含量和过氧化物酶同工酶谱带,证实与其亲本不同的上述三项特征都能通过再生传递在次级愈伤组织培养上表达。     

STUDIES ON 4-OXALYSINE-RESISTANT MUTANT CELL LINE AND ITS REGENERATED PLANT TISSUE FROM NICOTIANA TABACUM L.

A 4-oxalysine-resistant cell line from tobacco callus was selected and established through successive treatments with 1-4-oxalysine(OL, 1g/1), 2% EMS and then OL(4g/1). The mutant callus was not cross resistant to Eth and 5MT and accumulated twice amount of lysine as that of the parent. Mutant pleats were regenerated from the mutant cells. The mutant plants flowered and set seeds within the culture flasks. A secondary mutant cell line was induced from the mutant plant. The eL-resistance, lysine content and peroxidase isoenzyme of both the mutant cell and secondary cell line were examined and confirmed that all these traits, which were different from those of the parent line, were transmitted through the mutant cell line to its secondary callus. Experiment on sexual transmission is under way.