DEVELOPMENT OF COMMON WHEAT GERM-PLASM RESISTANT TO BARLEY YELLOW DWARF VIRUS BY BIOTECHNOLOGY

Barley yellow dwarf virus (BYDV) is one of the most serious wheat diseases in China. So far no resistance has been described in common wheat. A certain level of BYDV resistance was found in thirteen Triticeae species. Thinopyrum intermedium, two octoploids derived from TH. intermedium/wheat, Zhong 4 awnless and TAF46, and one disomic addition line, L1 derived from TAF46, showed good resistance to BYDV by enzyme linked immunosorbent assay (ELISA). Two wheat/TA. intermedium translocation lines, CPI 119880 and CPI 119899, showing good BYDV resistance were developed from L1 by using both CSph mutant and tissue culture. It is found that their BYDV resistance was controlled by a single dominant gene. Two cDNA probes pEleAcc3 and pPJN8 (E1-T1) were screened for detecting Th. intermedium DNA in wheat background. A specific band for the DNA of Th. intermedium and its derivatives was found in Southern hybridization. It is also possible to determine the size of the alien segment by comparing the relative density o     

应用生物技术向小麦导入黄矮病抗性的研究

小麦黄矮病是我国小麦的主要病害之一,迄今在普通小麦中还未发现抗性材料.经酶联免疫吸附分析,发现在小麦族中有13个种具不同程度抗性.中间偃麦草、小麦-中间偃麦草的八倍体衍生物无芒中4和TAF46及由TAF46育成的小麦附加系L_1均高抗黄矮病.以L_1为抗源,采用CSph突变体和组织培养诱导分别育成了抗病的易位系119880和119899.资料表明其抗性由一个显性基因所控制.筛选出pEleAcc3和pPJN8(E_1-T_1)两个互补DNA分子探针,可探测出在普通小麦遗传背景中的中间偃麦草DNA,在Southern杂交中,中间偃麦草及其衍生物的DNA清晰显示出小麦DNA所缺乏的一条特异带,通过比较其相对深浅程度可判断易位系所获的外源染色体片断的大小,作为黄矮病抗性的选择标志.