Energy transfer kinetics of phycoerythrocyanins (PECs) from the cyanobacterium Anabaena variabilis(Ⅰ)

The excitation energy transfer processes in nionomeric phycoerythrocyanins ( PEC)have been studied in detail using steady-state and time-resolved fluorescence spectra techniques as well as the deconvolution fech nique of spectra.The results indicate that the energy transfer processes should take place between α84 PVB md β8 or β155-PCB chromophores,the time constants of energy transfer are 34.7 and 130 ps individually;the component with lifetime of 1.57 ns originates from the fluorescence lifetime of the terminal emitter of β84 and /or β155 PCB chre-mophores; and the component with lifetime of 515 ps might be assigned to the energy transfer between two PCB chro mophores of β subunit.     

Excitation energy transfer mechanism in C-phycocyanin hexamer

ＢａｓｅｄｏｎｔｈｅｃｒｙｓｔａｌｓｔｒｕｃｔｕｒｅｄａｔａｆｒｏｍＸｒａｙａｎａｌｙｓｉｓ[1,2]ａｎｄｔｈｅｓｐｅｃｔｒａｌｐｒｏｐｅｒｔｉｅｓｏｆｅａｃｈｃｈｒｏｍｏｐｈｏｒｅｏｆＣＰＣ[3,4],Ｓａｕｅｒａｎｄｃｏｗｏｒｋｅｒｓ[5,6]ｅｘｐｅｃｔｅｄｔｈａｔｔｈｅｄｙｎａｍｉｃｓｏｆｅｎｅｒｇｙｔｒａｎｓｆｅｒｐｒｏｃｅｓｓｅｓｓｈｏｕｌｄｔａｋｅｐｌａｃｅｗｉｔｈｉｎ10—30ｐｓｔｉｍｅｓｃａｌｅｂｅｔｗｅｅｎｔｗｏｔｒｉｍｅｒｓｏｆＣＰＣｈｅｘａｍｅｒ.Ｆｕｒｔｈｅｒｍｏｒｅ,Ｚｈａｏａｎｄｃｏ…     

C-藻蓝蛋白六聚体内能量传递途径及其机制

利用皮秒级时间分辨荧光各向同性和各向异性光谱技术对C 藻蓝蛋白六聚体内的能量传递过程进行了比较详细的研究 .从实验上证实 :在孤立的六聚体内 ,连接 2个C PC三聚体的能量通道是由以 1 β15 5 6 β15 5 和 2α84 5α84为代表的两条途径来承担的 ,并且其能量传递的时间分别为 2 0和 1 0 ps左右 ;所探测到一个 45ps左右的时间常数来源于该六聚体内同一C PC三聚体内 3个简并β84 PCB发色团间的能量传递过程 .这些能量传递的作用机制可用F rster偶极 偶极机制来描述     

多变鱼腥藻中藻红蓝蛋白内能量传递机制的研究

通过利用稳态光谱技术,光谱解叠技术和时间分辨荧光光谱技术对PEC单体内的能量传递过程进行详细研究.结果表明:在PEC单体内.α亚基上的PVB发色团可以将激发能传递给β亚基上的2个PCB发色团上;其能量传递时间常数分别为34.7和130ps;1.57us的组分归属为β亚基上PCB发色团终端发射;515ps的组分可初步指认为β亚基上两个PCB发色团间的能量传递时间常数.