心脏手术中心肌组织高能磷酸化合物的高效液相色谱测定及比较研究

High-energy phosphate compounds from myocardial tissues were deternuned by HPLC with LichrosorbRP18 as stationary phase and phosphate buffer as mobile phase. The myocardial tissues were taken from car-diac patients whose hearts were perfused with cold crystalloid cardioplegia(CCC),cool blood cardioplegia(CBC)and warm blood cardioplegia(WBC) in cardiac surgery. The analytical results showed that CBC andWBC are superior to CCC in energy charge of myocardial tissue and will be used in cardiac surgery.