THE DISTRIBUTION OF P ELEMENTS IN D. melanogaster ALONG THE COAST OF CHINA STUDIED BY DNA HYBRIDIZATION METHOD

The distribution of the P-M system in four local Drosophila melanogaster populations obtained insouthern China has been examined by mating tests. There was no P strain. A HindⅢfragnment ofthe P element has been cloned, named as pPH 0.86, and used as probe in this study. The distribu-tion of the P element itself in thirteen local D. melanogaster populations along the eastern coast ofChina has been studied by the method of DNA hybridization. It is found that all the populations ob-tained from the north of Shanghai carry P elements but few populations from the south of Shanghai carry Pelements. In comparison with the mating test, the method of DNA hybridization is more direct andreliable.The origin and trends of distribution of P elements in China are discussed in this paper.     

应用DNA分子杂交研究黑腹果蝇P因子在中国沿海的分布

本文通过黑腹果蝇(Drosophila melanogaster)的交配试验研究了我国南部四个地区黑腹果蝇群体中P-M系统的分布,没有发现P品系。同时建立了P因子Hind Ⅲ片段克隆——pPH0.86。以此为探针,用DNA分子杂交法研究了我国沿海13个地区黑腹果蝇群体中P因子的分布,发现上海以北均带有P因子,上海以南则极少有P因子。比较了交配试验和DNA分子杂交两种方法,表明后一方法更为直观可靠。就P因子的传播途径,中国大陆P因子的来源及其传播趋向等作了讨论。