CLONING AND RESTRICTION MAPPING OF HUMAN HBV GENOME SEROTYPE adr

A Bam HI cleaved 3.2 Kb fragment from the HBV adr genome was cloned in E. coli, using pBR322 as vector. sixteen restriction sites from the action of Bam HI, Hind Ⅲ, Bgl Ⅰ, Bgl Ⅱ, Ava Ⅰ, Hinc Ⅱ, Sph Ⅰ, Xba Ⅰ, Xho Ⅰ and Sst Ⅱ were determined and mapped. No cleavage sites were found for Eco RI, Pst Ⅰ, Sma Ⅰ, Hpa Ⅰ, Kpn Ⅰ, Pvu Ⅱ and Sst Ⅰ.The restriction map for HBV adr is significantly different from those reported for the subtypes adw, ayw and adyw.     

人乙型肝炎病毒——HBVadr亚型基因组的克隆和限制酶切图谱

以pBR322为运载体将HBV adr亚型基因组Bam HI酶切的3.2Kb片段克隆入E.coli.Bam HI,HindⅢ,BglⅠ,BglⅡ,AvaⅠ,HincⅡ,SphⅠ,XbaⅠ,XhoⅠ,SstⅡ等10种限制酶的16个切点已被定位。制作了HBV adr亚型基因组的限制酶图谱。Eco RⅠ,PstⅠ,SmaⅠ,HpaⅠ,KpnⅠ,PvuⅡ,SstⅠ等没有切点,与已报道的adw,ayw,adyw亚型基因组的限制酶图谱明显不同。